Grace Catalog 2008

analytical hplc
hplc instruments
ELSD Sensitivity and Reproducibility
With reduced noise and greater baseline stability, the 3300 ELSD is more sensitive than any other ELSD available.
2
2
1
2
3
3
2
3
3
3
Competitor A
Competitor B
Competitor C
1. Methyl Paraben (200ng)
2. Propyl Paraben (200ng)
3. Butyl Paraben (200ng)
Model
Butyl Paraben
Limit of Detection (LOD)
3300 ELSD 25ng
Competitor A 200ng
Competitor B 200ng
Competitor C 150ng
Competitor D 50ng
Competitor D
1
3300 ELSD
0 1 2 3 4 5 6 7 8 Min.
Column: Alltech ® Alltima C18, 5µm, 4.6 x 250mm
(Part No. 88056)
Mobile Phase: Water:Acetonitrile (40:60)
Flow Rate: 1.0mL/min
Column Temp: Ambient
The 3300 ELSD’s near ambient temperature evaporation
maximizes sensitivity for semi-volatiles.
The 3300 ELSD is very reproducible as demonstrated by
10 identical injections of four ginkgolide components.
1
1
1. Ibuprofen (100ppm)
Component % RSD
1. Bilobalide 1.7
2. Ginkgolide C 1.3
3. Ginkgolide A 1.6
4. Ginkgolide B 1.0
1
1
2
1
30°C
3
4
32.5°C
1
35°C
37.5°C
1
40°C
42.5°C
0 1
2 3 Min.
0 2 4 6 8 10 12 14 16 Min.
Column: Alltech ® Alltima HP C18, 3µm, 2.1 x 100mm
(Part No. 87669)
Mobile Phase: Acetonitrile: 0.1%TFA in Water (80:20)
Flow Rate: 0.3mL/min
Column Temp: Ambient
Column: Alltech ® Platinum C18, 5µm, 4.6 x 250mm
(Part No. 32064)
Mobile Phase: Water:Methanol:THF (65:25:10)
Flow Rate: 1.0mL/min
Column Temp: Ambient
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