Grace Catalog 2008

hplc applications
0
Ginseng
1
2
Ginsenosides in Korean Ginseng
3
4
5
6
7 8 10
9
1. Rh2
2. Rh1
3. Rg2
4. Rg3
5. Rg1
6. Rf
11
7. Re
8. Rd
9. Rc
10. Rb2
11. Rb1
20 40 60 Min.
10142
Column: Alltech ® Prevail Carbohydrate ES, 5µm, 250 x 4.6mm
(Part No. 35101)
Mobile Phase: A: Acetonitrile:Water:Isopropyl Alcohol (80:5:15)
B: Acetonitrile:Water:Isopropyl Alcohol (67:21:12)
Gradient: Time: 0 28 35 45 50 51 57
%B: 25 85 80 75 90 100 25
Flow Rate: 0.8mL/min
Detector: ELSD
600000
500000
400000
300000
200000
100000
0
-100000
40
12
50 60 70 80 90 100 Min.
Competitor 1 C18
Competitor 2 C18
Competitor 3 C18
Ginseneoside
3
1. Rg1
2. Re
3. Rb1
10765
Competitor 4 C18
Competitor 5 C18
GraceSmart C18
Column: GraceSmart C18, 5µm, 250 x 4.6mm (Part No. 5138810)
Mobile Phase: A: Water B: Acetonitrile
Gradient: Time: 0 35 55 70 100
%B: 19 19 29 29 40
Flow Rate: 1.0mL/min
Detector: ELSD
nutraceuticals | vitamins
Ginkgo Biloba
Folium Ginkgo
Terpene Lactones High Throughput
10742
10288
2
1
3
4
1. Ginkgolide C
2. Bilobalide
3. Ginkgolide A
4. Ginkgolide B
1. Bilobalide
2. Ginkgolide J
3. Ginkgolide C
4. Ginkgolide A
5. Ginkgolide B
1 2 3
4 5
0 2.5 5 7.5 10 12.5 15 17.5 20 22.5 25 Min.
0
1 2 3 4 5 6 Min.
Column: GraceSmart C18, 5µm, 250 x 4.6mm (Part No. 5138810)
Mobile Phase: Methanol:Tetrahydrofuran:Water (25:10:65)
Flow Rate: 1.0mL/min
Detector: ELSD
Column: Alltech ® Alltima C18, 3µm, 53 x 7mm Rocket
(Part No. 50605)
Mobile Phase: A: 0.1% Trifluoroacetic Acid in Water
B: 0.1% Trifluoroacetic Acid in Methanol
Gradient: Time: 0 5
%B: 10 45
Flow Rate: 5.0mL/min
Detector: ELSD
Ginkgo Biloba Flavonol Glycoside Standards
Ginkgo Biloba Terpene Lactone Standards
1
4
10239 10287
2
3
1. Quercetin
2. Kaempferol
3. Isorhamnetin
1
23
5
1. Bilobalide
2. Ginkgolide J
3. Ginkgolide C
4. Ginkgolide A
5. Ginkgolide B
0
2 4 6 8 10 12 14 16 18 20 Min.
Column: Alltech ® Platinum C18, 5µm, 250 x 4.6mm
(Part No. 32064)
Mobile Phase: A: 0.05% Trifluoroacetic Acid in Water:Methanol (95:5)
B: 0.05% Trifluoroacetic Acid in Methanol
Gradient: Time: 0 20
%B: 10 90
Flow Rate: 1.0mL/min
Detector: ELSD
0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min.
Column: Alltech ® Alltima C18, 3µm, 100 x 4.6mm
(Part No. 813812)
Mobile Phase: A: 0.1% Trifluoroacetic Acid in Water
B: 0.1% Trifluoroacetic Acid in Methanol
Gradient: Time: 0 15
%B: 10 55
Flow Rate: 1.3mL/min
Detector: ELSD
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