Grace Catalog 2008

hplc applications
Bacitracin
Bacitracin
0 1 2 3 4 5 6 7 8 Min.
0 1
Spectinomycin
1
1. Spectinomycin
2 3 4 5 6 7 8 9 Min.
10680
pharmaceuticals
Column:
GENESIS Jones FM15960E Genesis ® (C18, C18, 4 4µm, 4.6150 mm x i.d. 4.6mm x 150 mm)
Flow:
1.0 mL/min (Part No. FM15960E)
Mobile Eluent: Phase: 31% Acetonitrile:0.1% ACN/0.1% TFA in water Trifluoroacetic Acid (31:69)
Flow Detection: Rate: Absorbance, 1.0mL/min 214 nm
Detector: UV at 214nm
Column: Alltech ® Alltima HP C18 Amide, 3µm, 50 x 4.6mm
(Part No. 87829)
Mobile Phase: 10mM Ammonium Carbonate:Acetonitrile (98:2)
Flow Rate: 1.0mL/min
Detector: ELSD
mAU
100
80
60
40
Clarithromycin on
Chinese Pharmacopeia Assay
1. Clarithromycin
10760
mAU
500
400
300
200
Clindamycin Hydrochloride on
Chinese Pharmacopeia Assay
1. Clindamycin
10768
20
100
0
0
2 4 6 8 10 12 14 16 18 Min.
Competitor 1 C18
Competitor 4 C18
Competitor 2 C18
Competitor 5 C18
Competitor 3 C18
GraceSmart C18
Column: GraceSmart C18, 5µm, 250 x 4.6mm (Part No. 5138810)
Mobile Phase: Phosphate Buffer (Dissolve 9.11g Potassium Dihydrogen
Phosphate in 1000mL Water, add 2mL Triethylamine, adjust
with Phosphoric Acid to pH of 5.5):Acetonitrile (60:40)
Flow Rate: 1.0mL/min
Detector: UV at 210nm
0
0 2 4 6 8 10 12 14 Min.
Competitor 1 C18
Competitor 4 C18
Competitor 2 C18
Competitor 5 C18
Competitor 3 C18
GraceSmart C18
Column: GraceSmart C18, 5µm, 250 x 4.6mm (Part No. 5138810)
Mobile Phase: Methanol:Ammonium Dihydrogen Phosphate Solution
(Dissolve 2.88g Ammonium Dihydrogen Phosphate in
1000mL Water, adjust with Phosphoric Acid to pH3.0)
300:210
Flow Rate: 1.0mL/min
Detector: UV at 214nm
mAU
1400
1200
1000
800
600
400
200
0
0
Ceftriaxone Sodium on
Chinese Pharmacopeia Assay
1. Ceftriaxone
5 10 15 20 25 Min.
Competitor 1 C18
Competitor 2 C18
Competitor 3 C18
10776
Competitor 4 C18
Competitor 5 C18
GraceSmart C18
Column: GraceSmart C18, 5µm, 250 x 4.6mm (Part No. 5138810)
Mobile Phase: 0.02mol/L n-Octylamine:Acetonitrile (73:27)
Flow Rate: 1.0mL/min
Detector: UV at 254nm
mAU
700
600
500
400
300
200
100
0
0
Cefradine on
Chinese Pharmacopeia Assay
1. Ceftriaxone
5 10 15 20 25 30 35 40 45 50 Min.
Competitor 1 C18
Competitor 2 C18
Competitor 3 C18
10767
Competitor 4 C18
Competitor 5 C18
GraceSmart C18
Column: GraceSmart C18, 5µm, 250 x 4.6mm (Part No. 5138810)
Mobile Phase: Water:Methanol:3.86% Sodium Acetate:4% Acetic Acid
(1564:400:30:6)
Flow Rate: 0.9mL/min
Detector: UV at 254nm
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