Grace Catalog 2008

hplc applications
Barbiturates
2
4
1 3
5
Barbiturate Mix
1. Primidone
2. Metharbital
3. Mephenytoin
4. Phenobarbital
5. Phenytoin
10164
Anticonvulsants
Anticonvulsants
3
4
2
1
1. Ethotoin
2. Mephenytoin
3. Carbamazepine
4. Diazepam
9394
pharmaceuticals
0 2
4 6 8 10 12 Min. 0 2
4 6 8 Min.
Column: ZirChrom ® DiamondBond-C18, 3µm, 100 x 4.6mm
(Part No. DB01-1046)
Mobile Phase: 0.05M Ammonium Phosphate, pH7.0:Tetrahydrofuran
(75:25)
Flow Rate: 1.0mL/min
Detector: UV at 220
Column: Alltech ® Prevail Phenyl, 5µm, 150 x 4.6mm
(Part No. 99239)
Mobile Phase: A: Water B: Acetonitrile
Gradient: Time: 0 15
%B: 25 60
Flow Rate: 1.5mL/min
Detector: UV at 254
Anticonvulsants
Anticonvulsants
10348 10349
1
2
1. Ethotoin
2. Phenytoin
3. Mephenytoin
4. Carbamazepine
3 4 0 2 4 6 8 10 12 14 16 18 20 Min.
1
2
3
4
1. Ethotoin
2. Phenytoin
3. Mephenytoin
4. Carbamazepine
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Min.
Column: Alltech ® Alltima HP EPS C18, 5µm, 150 x 4.6mm
(Part No. 87715)
Mobile Phase: 0.025M Ammonium Acetate:Acetonitrile (70:30)
Flow Rate: 1.0mL/min
Detector: ELSD
Column: Alltech ® Alltima HP C18 HiLoad, 5µm, 150 x 4.6mm
(Part No. 87697)
Mobile Phase: 0.025M Ammonium Acetate:Acetonitrile (70:30)
Anti-Anginal Drugs
Flow Rate: 1.0mL/min
Detector: ELSD
Anti-Anginals
Anticonvulsants
Anticonvulsants
2
3
1. Uracil
2. Carbamazepine
3. Phenytoin
1
2
Anti-Anginal Drugs
3
4
1. Atenolol
2. Metoprolol
3. Propranolol
4. Diltiazem
1
0 1 2 3 4 5 Min.
0 1 2 3 4 5 Min.
Column: Jones Genesis ® Phenyl, 4µm, 50 x 4.6mm
Column: GENESIS FM5980E (Phenyl, 4 µm, 4.6 mm i.d. x 50 mm)
(Part No. FM5980E)
Flow:
1.0 mL/min
Mobile Eluent: Phase: Isocratic, Methanol:Water 30% MeOH in (30:70) water
Flow Detection: Rate: Absorbance, 1.0mL/min 210 nm
Detector:
Peaks: 1. Uracil, UV at 2. 210nm Carbamazepine, 3. Phenytoin
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Column: Column: GENESIS Jones Genesis FM10960E
® C18, 4µm, 100 x 4.6mm
(C18, (Part 4 No. µm, FM10960E)
4.6 mm i.d. x 100 mm)
Mobile Flow: Phase: 1.0 Acetonitrile:50mM mL/min
KH2PO4, pH3.0 (40:60)
Flow Eluent: Rate: Isocratic, 1.0mL/min 40% ACN in 50 mM KH 2 PO 4 , pH 3.0
Detector: Detection: Absorbance, UV at 254nm 254 nm
Peaks: 1. Atenolol, 2. Metoprolol, 3. Propranolol, 4. Diltiazem
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