Grace Catalog 2008

prep | flash | tlc
GraceAlpha Columns
A New Silica Generation
new
• High-porosity surface increases mass transfer and results
in increased column efficiencies and loading capacity
• Dense core and highly spherical shape yield
mechanically robust particle
• Proprietary bonding technology gives unique selectivity
and increased resolution
GraceAlpha Performance
Outstanding Loading Capacity
GraceAlpha silica has a high-porosity exterior yielding
higher loading capacities than conventional silicas.
Unique Selectivity
Proprietary bonding chemistry provides unique selectivity that
yields advantages not seen in other C18 and C8 phases.
prep | columns
Norm.
800
600
400
200
Insulin Frontal Loading Capacity
on C8 Columns with 0.1% TFA
Insulin loading capacity is 16 to 1000% higher recovery on
the GraceAlpha C8 column.
Competitor A
14 mg/mL
Competitor B
133 mg/mL GraceAlpha
154 mg/mL
Peptide Standard Mixture
The GraceAlpha column offers selectivity advantages.
1
1
2
2
3 4
5
3 4
5
GraceAlpha
Column
Competitor
1. GY (238 Da)
2. VYV (379 Da)
3. Met Enkephalin
(YGGFM, 573 Da)
4. Angiotensin II
(DRVYIHPF, 1045 Da)
5. Leu Enkephalin
(YGGFL, 555 Da)
0
0 20 40 60 80 100 120 140 160 180
Breakthrough Time (min.)
High Resolution and Recovery
GraceAlpha columns increase purity and yield of synthetic
peptides with superior resolution of impurities due to higher
mass transfer and optimized C18 and C8 bonding chemistry.
Crude 20-AA Synthetic Peptide
The target peptide is better resolved from impurities, with
33% higher recovery, on the GraceAlpha column.
0 6 12 18 24 30 Min.
Columns: All C18, 5µm, 4.6 x 250mm
Mobile Phase: A: 0.1% TFA in Water, B: 0.085% TFA in Acetonitrile
Gradient: Time: 0 30 35 40
%B: 10 40 90 90
Flow Rate: 1.0mL/min
Detector: UV at 225nm
Column Temp.: 25°C
Resolution (USP)*
Peptide Peak GraceAlpha Column Competitor
1 42.0 35.7
2 32.2 28.3
3 6.0 2.3
4 7.4 11.3
*Resolution is based on the next adjacent peak.
Better resolution
of closely eluting
impurities
0.00 5.00 10.00 15.00 19.99
Competitor
Column 4µm
GraceAlpha
Column 5µm
Columns: All C18, 4.6 x 250mm
Mobile Phase: A: 0.1% TFA in Water, B: 0.1% TFA in Acetonitrile
Gradient: Time: 0 20
%B: 20 40
Flow Rate: 1.0mL/min
Data courtesy of: Biopeptide Inc.
related products
GraceAlpha bulk media is also
available for scale-up. See page
176 for more information.
7250
152 www.discoverysciences.com