Grace Catalog 2008

analytical hplc
hplc columns | small molecule
GraceAlpha Columns (Patent Pending)
A New Silica Generation
• High-porosity surface increases mass transfer and results in increased column
efficiencies and loading capacity
• Dense core and highly spherical shape yields mechanically robust particle
• Proprietary bonding technology gives unique selectivity and increased resolution
• Ideally suited for scale up to prep applications
Extreme Porosity
High-porosity exterior increases mass transfer leading to higher
efficiencies and superior loading capacity.
The high-porosity structure adds an elastic quality to the silica surface
allowing the particles to pack tighter yielding higher efficiencies. It also
stabilizes the packed bed preventing voids.
Dense Inner Core
new
Dense core provides exceptional strength. Typically the high-porosity
structure would result in a weak particle prone to breaking, but the
GraceAlpha particle combines the high porosity outer region with a dense
inner core that results in a particle that resists breaking and forming fines
that contribute to high backpressures.
GraceAlpha Phase Specifications
Phase Base Material Particle Shape Particle Size Pore Size Surface Area Carbon Load Phase Type Endcapped? USP L-code
C18 Silica Spherical 5, 10, 15, 20µm 120Å 325m 2 /g 15% Monomeric Yes L1
C8 Silica Spherical 5, 10, 15, 20µm 120Å 325m 2 /g 10% Monomeric — L7
Silica Silica Spherical 5, 10, 15, 20µm 120Å 325m 2 /g — Monomeric — L3
High Level of Resolution
GraceAlpha offers resolution advantages due to its higher
mass transfer and optimized C18 and C8 bonding chemistry.
Adsorbence
Adsorbence
1.0
0.5
0
1.0
0.5
0
0
0
Crude 20-AA Synthetic Peptide
from Biopeptide Co., Inc.
Better resolution
of closely
eluting impurities
GraceAlpha Column 5µm
5
Competitor 4µm
5
10 15 19.99 Min.
10 15 19.99 Min.
GraceAlpha Columns
Packing i.d. x Length Part No.
C18, 5µm 4.6 x 150mm 5140743
4.6 x 250mm 5140702
C18, 10µm 4.6 x 150mm 5140744
4.6 x 250mm 5140701
C18, 15µm 4.6 x 150mm 5141058
4.6 x 250mm 5141059
C8, 5µm 4.6 x 150mm 5140740
4.6 x 250mm 5140704
C8, 10µm 4.6 x 150mm 5140742
4.6 x 250mm 5140703
C8, 15µm 4.6 x 150mm 5141055
4.6 x 250mm 5141057
Silica, 5µm 4.6 x 150mm 5140740
4.6 x 250mm 5140704
Silica, 10µm 4.6 x 150mm 5140742
4.6 x 250mm 5140703
Silica, 15µm 4.6 x 150mm 5141055
4.6 x 250mm 5141057
Columns: All C18 4.6 x 250mm
Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile
Gradient: Time: 0 20
%B: 20 40
Flow Rate: 1.0mL/min
The target peptide is better resolved from impurities, with 33% higher
recovery, on the GraceAlpha column.
more applications
To view our complete searchable
chromatogram database visit
www.discoverysciences.com/chromdb/
52
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