Grace Catalog 2008

hplc applications
pharmaceuticals
Diuretics
2
1
4
3
10
7
5 6 8 9
12
11
13
14
15
Diuretics and Uricosuric Agents
1. Amiloride HCl Hydrate
2. Acetazolamide
3. Hydrochlorothiazide
4. Triamterene
5. Chlorthalidone
6. Furosemide
7. Hydroflumethiazide
8. Clopamide
9. Ethacrynic Acid
10. Trichloromethiazide
11. Probenecid
12. Indapamide
13. Bumetanide
14. Bendroflumethiazide
15. Spironolactone
10246
0
5 10 15 20 25 30 Min.
Column: Alltech ® Alltima C18, 5µm, 250 x 4.6mm (Part No. 88056)
Mobile Phase: A: 0.1% Trifluoroacetic Acid in 0.025M Ammonium Acetate B: 0.1% Trifluoroacetic Acid in Acetonitrile
Gradient: Time: 0 30
%B: 25 65
11
Flow Rate: 1.0mL/min
Detector: UV at 254nm
10
1
2 3
4
5
6
7
8
9
12
Doping Agents
1. Amiloride HCl Hydrate
2. Acetazolamide
3. Hydrochlorothiazide
4. Chlorthalidone
5. Hydroflumethiazide
6. Clopamide
7. Trichloromethiazide
8. Indapamide
9. Bendroflumethiazide
10. Bumetanide
11. Probenecid
12. Spironolactone
10262
0
1 2 3 4 5 6 7 8 9 10 11 12 Min.
7
Column: Alltech ® Alltima C18, 5µm, 250 x 4.6mm (Part No. 88056)
Mobile Phase: A: 0.1% Trifluoroacetic Acid in 0.025M Ammonium Acetate B: 0.1% Trifluoroacetic Acid in Acetonitrile
Gradient: Time: 0 10 15
%B: 25 95 95
Flow Rate: 1.0mL/min
3
5 6 6. Trichloromethiazide
7. Indapamide
Detector: ELSD
8
Diuretics — High Throughput
1. Acetazolamide
2. Hydrochlorothiazide
10265
1
9
3. Triamterene
4. Hydroflumethiazide
5. Clopamide
8. Bumetanide
9. Spironolactone
2 4
0
1 2 3 4 Min.
Column: Alltech ® Alltima C18, 3µm, 53 x 7mm Rocket (Part No. 50605)
Mobile Phase: A: 0.1% Trifluoroacetic Acid in 0.025M Ammonium Acetate B: 0.1% Trifluoroacetic Acid in Acetonitrile
Gradient: Time: 0 4
%B: 20 95
Flow Rate: 3.5mL/min
Detector: UV at 254nm
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