Grace Catalog 2008

hplc applications
Anesthetics
1 + 2
3
Basic Pharmaceuticals
1. Procaine
2. Lidocaine
3. Tetracaine
1 2
Basic Pharmaceuticals
10737 10738
3
1. Procaine
2. Lidocaine
3. Tetracaine
pharmaceuticals
0 0.5 1 1.5 2 2.5 3 Min.
Column: Grace ® VisionHT C18, 1.5μm, 50 x 2.0mm
(Part No. 5139605)
Mobile Phase: 25mM Potassium Phosphate, pH7.0:Methanol (30:70)
Flow Rate: 0.5mL/min
Detector: UV at 230nm
0 0.5 1 1.5 2 2.5 3 Min.
Column: Grace ® VisionHT C18-P, 1.5μm, 50 x 2.0mm
(Part No. 5139606)
Mobile Phase: 25mM Potassium Phosphate, pH7.0:Methanol (30:70)
Flow Rate: 0.5mL/min
Detector: UV at 230nm
Anesthetic Mix
Anesthetics
Anesthetics
4
10118
3
1
2
3
5
1. p-Aminobenzoic Acid
2. Benzocaine
3. Tetracaine
4. Dibucaine
5. Butacaine
1
2
4
5
6
1. Procaine
2. Benzocaine
3. Mepivacaine
4. Lidocaine
5. Tetracaine
6. Dibucaine
0
2 4 6 8 10 12 Min.
3
0 10 20 30 40 50 60 Min.
Column: Alltech ® Alltima C8, 5µm, 150 x 4.6mm (Part No. 88072) Column: Vydac ® Denali ® , 5µm, 150 x 4.6mm (Part No. 238DE5415)
Mobile Phase: A: Water B: Methanol C: Acetonitrile
Mobile Phase: Methanol:50mM K2HPO4 in Water, pH7.0 (60:40)
Gradient: Time: 0 7
Column: Flow Rate: DENALI 238DE5415 1.0mL/min (5 µm, 4.6 mm i.d. x 150 mm)
%B: 15 45
Flow:
Detector:
1.0 mL/min
UV at 220nm
Eluent: Isocratic, 60:40 MeOH:H 2 O containing
%C: 15 35
50 mM K 2 HPO 4 , pH 7
Flow Rate: 1.5mL/min
Temp: 30 °C
Detector: UV at 230nm
Detection: Absorbance, 220 nm
Peaks: 1. Procaine, 2. Benzocaine, 3. Mepivacaine,
4. Lidocaine, 5. Tetracaine, 6. Dibucaine
Opiates
Opiates
10165
Opiates
1
2
4
1. Naloxone
2. Codeine
3. Ethylmorphine
4. Oxycodone
1
3
Opiates
1. Hydromorphone
2. Oxycodone
3. Codeine
4. Ethylmorphine
5. Nalorphine
2
4
5
0 1 2 3 4 5 Min. 0 5 10 15 20 25 Min.
Column: ZirChrom ® DiamondBond-C18, 3µm, 100 x 4.6mm Column: Vydac ® Denali ® , 5µm, 250 x 4.6mm (Part No. 238DE54)
(Part No. DB01-1046)
Mobile Column: Phase: DENALI Methanol:20mM 238DE54 (5 µm, K2HPO4 4.6 mm in i.d. Water, x 250 mm) pH7.0 (50:50)
Mobile Phase: 0.02M Ammonium Phosphate, pH11.0:
Flow: Rate: 1.0 1.0mL/min
Tetrahydrofuran (73.5:26.5)
Detector:
Eluent: Isocratic,
UV at
50:50
254
MeOH:H 2 O containing
20 mM K 2 HPO 4 , pH 7
Flow Rate: 1.0mL/min
Temp: Ambient at 22 °C
Detector: UV at 220
Detection: Absorbance, 254 nm
Sample: 10 µL containing 10 µg of each analyte
more applications
Peaks: 1.Hydromorphone, 2. Oxycodone, 3. Codeine,
View our complete searchable chromatogram database at www.discoverysciences.com/chromdb/
4. Ethylmorphine, 5. Nalorphine ww.discoverysciences.com
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