Grace Catalog 2008

analytical hplc
ELSD Performance Benefits
Stable Baselines with Fast, Steep Gradients
1
0 1 2 3 Min.
2
3
10658
1. Acetylsalicylic Acid
2. Diphenhydramine
3. Trimipramine
Use ELSD to Trigger Collection of UV
Transparent Compounds in Prep Applications
10679
1. LLLn
2 3
2. LLL
3. LLO
4. PLL
4 5. LOO
5 6. PLO
6 7. PLP
7,8 8. OOO
1
9,10
9. SLO
10. POO
0 5 10 15 20 Min.
hplc instruments
Column: Alltech ® Alltima HP C18, 3µm,
7 x 33mm Rocket (Part No. 87671)
Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile
Gradient: Time: 0 3
%B: 10 90
Flow Rate: 2.5mL/min
Column Temp: 30°C
Simplify Detection of Weak Absorbing Polymers
Column: Vydac ® Denali ® 238DE C18, 10µm, 22 x 150mm
(Part No. 238DE102215)
Mobile Phase: A: Acetonitrile B: Dichloromethane
Gradient: Time: 0 10 20 21
%B: 45 50 70 45
Flow Rate: 10mL/min
Column Temp: 25°C
Easily Detect Non-Chromophoric Drug Compounds
1
10636
10680
1. mPEG-SBA
1
1. Spectinomycin
0 3 6 9 12 15 Min.
Column: Alltech ® ProSphere 300, 5µm, 4.6 x 150mm
(Part No. 35560)
Mobile Phase: A: Water B: Acetonitrile
Gradient: Time: 0 3 15
%B: 30 30 80
Flow Rate: 1.0mL/min
Column Temp: Ambient
0 1
2 3 4 5 6 7 8 9 Min.
Column: Alltech ® Alltima HP C18 Amide, 3µm, 4.6 x 50mm
(Part No. 87829)
Mobile Phase: 10mM Ammonium Carbonate:Acetonitrile (98:2)
Flow Rate: 1.0mL/min
Column Temp: Ambient
Use ELSD to Simplify Triglyceride Analysis
600
500
400
300
200
100
0
0 5 10
1
Sesame Seed Oil
2
3
4
5 7 8 9
6
10
15 20 25 30 35 Min.
1. LLL
2. LLO
3. LLP
4. OOL
5. POL
6. PPL
7. OOO
8. OOP
9. PPO
10. OOS
Column: Alltech ® Alltima HP C18 Hi-Load, 5µm,
4.6 x 250mm (Part No. 87698)
Mobile Phase: A: Acetonitrile B: Dichloromethane
Gradient: Time: 0 25 26 27 28
%B: 30 51 70 70 30
Flow Rate: 1.0mL/min
Column Temp: 20°C
10581
5 6
Improve Sensitivity for Carbohydrates
Robitussin ® Flu Syrup
10557
1. Sucralose
2 4
2. Fructose
3. Sorbitol
4. Glucose
5. Sucrose
3
6. Maltose
1
0 5 10 15 20 25 30 35 40 Min.
Column: Alltech ® Prevail Carbohydrate ES, 5µm,
4.6 x 250mm (Part No. 35101)
Mobile Phase: A: Acetonitrile B: Water
Gradient: Time: 0 5 10 15 25
%B: 15 15 25 25 50
Flow Rate: 1.0mL/min
Column Temp: Ambient
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