Grace Catalog 2008

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analytical hplc hplc columns | introduction High Throughput HPLC Introduction The Benefits of <strong>Grace</strong> ® Small Particles and Formats Today’s laboratories are under greater demand to analyze more samples in less time. To help meet this demand, major product advances have been made to decrease analysis time and increase HPLC throughput. HPLC system advances have greatly reduced the influence of extra column volumes and have extended the range of flow rates and pressure capabilities. In response to this, <strong>Grace</strong> now offers a wide range of column chemistries and formats appropriate for all types of high throughput systems. The use of smaller particles offers two main improvements to the chromatographic separation—increased resolution and speed. Resolution is directly proportional to the square root of column efficiency, therefore the higher the efficiency, the narrower the peaks and the greater the resolution between them. Increased speed comes from higher mobile phase flow rates that can be used without loss in efficiency, and higher flow rates mean faster analysis times. Understanding the type of system currently in use for high throughput separations and then matching the right column configuration is critical to achieving the best high throughput separation. Here we outline the three types of systems currently in use today for high throughput and the recommended HPLC column format for use with that system. System Type 1 Ultra High-Pressure LC System (>10,000psig pressure limitation) Examples: Agilent 1200, Waters ® Acquity ® , Thermo Accela, Jasco XLc. Speed from Ultra High-Pressure Systems: Representing the latest in LC instrumentation technology, ultra high-pressure systems theoretically have the potential to deliver the fastest separations. They have minimal system volume and offer a pressure limit upwards of 12,000psig. This allows the use of columns with sub 2µm particles and 2–7 times traditional flow rates. Sub 2µm particles extend the working range of acceptable mobile phase linear velocities without sacrificing efficiency. Therefore, you can push flow rates and still get equal or better performance. Suggested Column Format: VisionHT columns are designed for microbore and ultra high-pressure LC systems that have small system volumes to limit peak broadening from sample diffusion. VisionHT columns are packed with highly efficient 1.5µm phases that yield taller peaks and increase sensitivity. The column hardware incorporates a low dead volume design to minimize sample diffusion, and maintain peak integrity and efficiency. 12,000psig high-pressure stability allows fast flow rates, decreasing run times 10 fold. System Type 1 Recommended Column Format: Length: i.d.: VisionHT 20, 30, 50, 100mm 1.0 and 2.0mm tech tip Converting Methods From Traditional Column Formats to High Throughput Columns 7135 Convert Between Standard HPLC and VisionHT Columns Flow Rate Standard HPLC (4.6mm) 1.0X VisionHT (2.0mm) 2.3X When adjusting between standard LC conditions to VisionHT columns convert flow rates accordingly and then increase flow rate for faster analysis. Convert Between Standard HPLC Columns and Rocket Columns Flow Rate Standard Analytical (4.6mm) 1.0X Rocket Column (7.0mm) 2.3X mAU 20 15 10 5 0 VisionHT Columns for Ultra High-Pressure LC 2 5 1 3 4 6 7 8 10724 1. Sulfadiazine 2. Sulfathiazole 3. Sulfamerazine 4. Sulfamethazine 5. Sulfamethoxazole 6. Sulfaisoxazole 7. Sulfaquinoxilone 8. Sulfamethoxine Use this conversion of flow rate to transfer methods between Rocket column or VisionHT columns. Backpressure on standard LC systems should be considered. 0 1 2 3 4 5 Min. System Agilent 1200 Mobile Phase: A: 0.1% Formic Acid B: Methanol Detector: UV at 280nm Temperature: Ambient more info See pages 34–37 for more information on VisionHT columns. 32 www.discoverysciences.com
analytical hplc System Type 2 Low Volume, High Throughput (HTP) LC System (
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analytical hplc Vydac ® MS Columns
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analytical hplc Vydac ® Everest ®
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analytical hplc Vydac ® 214TP C4 V
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analytical hplc Vydac ® ProZap C1
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analytical hplc Vydac ® Venture ®
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analytical hplc Alltech ® ProSpher
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analytical hplc Alltech ® Anion Co
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analytical hplc Alltech ® Anion Co
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analytical hplc Hamilton ® Anion,
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analytical hplc Alltech ® Surfacta
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analytical hplc Empty HPLC Hardware
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analytical hplc Stainless Steel Fri
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analytical hplc Prefilters Direct-C
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analytical hplc Two-Piece High-Pres
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analytical hplc High-Pressure Adapt
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analytical hplc High- and Low-Press
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analytical hplc Low-Pressure Adapte
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analytical hplc Fitting and Accesso
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analytical hplc Mobile Phase Inlet
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analytical hplc Mobile Phase Delive
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analytical hplc Kontes Ultra-Ware
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analytical hplc Alltech ® Online D
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analytical hplc Alltech ® Adjustab
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analytical hplc ASI Pump Replacemen
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analytical hplc Hamilton ® Priming
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analytical hplc Rheodyne ® Model 8
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analytical hplc Rheodyne ® RheBuil
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analytical hplc Rheodyne ® ChromTR
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analytical hplc Alltech ® SelectPR
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analytical hplc Hamilton ® Miniatu
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Prep | Flash | TLC Preparative Chro
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prep | flash | tlc Vydac ® MS 300
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prep | flash | tlc Vydac ® TP Colu
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prep | flash | tlc Alltech ® Prep
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prep | flash | tlc Spring Column H
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prep | flash | tlc Axial SFC Column
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prep | flash | tlc Axial SFC Column
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prep | flash | tlc Davisil ® Media
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prep | flash | tlc Davisil ® Media
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prep | flash | tlc Pumps, Injectors
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prep | flash | tlc GraceResolv Sil
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prep | flash | tlc TLC Plates Davis
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GC GC Instruments. . . . . . . . .
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gas chromatography Preconfigured SR
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gas chromatography SRI Accessories
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gas chromatography PeakSimple for
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gas chromatography Hydrogen Generat
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gas chromatography Zero Air Generat
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gas chromatography JUN-AIR ® Oil-L
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gas chromatography Grace ® GC Capi
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gas chromatography Alltech ® Custo
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gas chromatography Alltech ® GC Pa
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gas chromatography Packing Accessor
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gas chromatography Flowmeters Aalbo
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gas chromatography Gas Leak Detecti
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gas chromatography Physical Charact
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gas chromatography Oxygen Traps Pro
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gas chromatography Alltech ® Gas P
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gas chromatography Purifier Accesso
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gas chromatography Gas Sampling Bag
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gas chromatography Gas Sampling Acc
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gas chromatography GC Ferrules (con
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gas chromatography Parker Fittings
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gas chromatography Swagelok ® Fitt
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General Chromatography SPE . . . .
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general chromatography Alltech ® S
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general chromatography Alltech ® E
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general chromatography Alltech ® M
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general chromatography Alltech ® M
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general chromatography GracePure S
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general chromatography Manifold Acc
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general chromatography Filtration I
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general chromatography Recommended
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general chromatography Individual S
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general chromatography Gas Standard
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general chromatography Special Gas
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general chromatography Hydrocarbons
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general chromatography Popper Glass
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general chromatography Silanized Gl
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general chromatography Screw Thread
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general chromatography Polypropylen
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general chromatography Crimpers and
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general chromatography Flex-Connect
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general chromatography Low-Pressure
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general chromatography Glass-Lined
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hplc applications Analgesics Anti-I
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hplc applications Antidepressants T
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hplc applications Barbiturates 2 4
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hplc applications Anesthetics 1 + 2
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hplc applications Anti-Asthmatics M
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hplc applications Antibiotics Antib
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hplc applications Bacitracin Bacitr
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hplc applications Taxols Taxol ® a
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hplc applications 0 Ginseng 1 2 Gin
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hplc applications Chinese Pharmacop
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hplc applications Vitamins 1 2 3 5
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hplc applications 1 2 b-Carotenes 3
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hplc applications Carbohydrates 1 C
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hplc applications 3 1 2 4 Conquista
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hplc applications Organic Acids 1 8
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hplc applications Oils / Lipids 2 S
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hplc applications Oils / Lipids 1 2
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hplc applications Proteins & Peptid
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hplc applications Purification of T
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hplc applications 1) Cystine 2) Cys
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hplc applications Miscellaneous Pho
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hplc applications 1 Organochlorine
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hplc applications Water Pollutants
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hplc applications Polymers Polyuret
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hplc applications Biofuels Triacylg
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hplc applications Fuels Petrochemic
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ic applications 2 Anions 13033 2 Ba
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ic applications 3 Anions with pHBA
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ic applications Cations Rapid Analy
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ic applications Surfactants 1 2 Sho
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gc applications Ephedra Standards v
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gc applications Organic Volatile Im
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gc applications Fast GC Drug Analys
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gc applications
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gc applications Semivolatiles Colu
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gc applications Volatiles 5,6 Aroma
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gc applications EPA Method 624 —
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gc applications Alcohols The Glenli
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gc applications Essential Oils 1 2
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gc applications FAMEs 1 3 4 5 Sesam
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gc applications Fuel Texas Crude Oi
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gc applications Gases Scott Mix 216
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gc applications Amines Polyamines 2
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gc applications Gases 1 5 MAPP Gas
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gc applications Solvents Volatile O
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gc applications Test Mixes AT-
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spe applications Diuretics Procedur
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spe applications Additives Procedur
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spe applications Pesticides Procedu
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spe applications PAHs Procedure usi
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Grace Catalog 2008

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