Grace Catalog 2008

hplc applications
Antidepressants
Tricyclic Amines
Alprazolam and Triazolam by the USP XXII Method
1
2
3
1. Fluphenazine HCl
2. Chlorpromazine HCl
3. Phenothiazine HCl
9105
1
2
1. Triazolam
2. Alprazolam
7368
pharmaceuticals
0 1
2
3
2
4 5 6 7 8 Min.
Column: Alltech ® Alltima CN, 3µm, 150 x 4.6mm
(Part No. 81189)
Mobile Phase: 0.01M KH2PO4, pH2.6:Acetonitrile (50:50)
Flow Rate: 1.0mL/min
Detector: UV at 254nm
3
Basic Drugs
1. Diphenhydramine
2. Nortriptyline
3. Amitriptyline
10353
0 2 4 6 8 10 12 14 16 18 20 Min.
Column:
Mobile Phase:
Flow Rate:
Detector:
1
2
Alltech ® Alltima Silica, 5µm, 300 x 4.6mm (Custom)
Acetonitrile:Chloroform:i-Butyl Alcohol:Water:Glacial
Acetic Acid (85:8:5:2:0.05)
2.0mL/min
UV at 254nm
Tricyclic Antidepressants
4
1. Desipramine
2. Nortryptyline
3. Diphenhydramine
4. Amitriptyline
10373
1
3
0 1 2 3 4 5 6 7 8 9 Min.
0
2 4 6 8 10 12 14 16 18 20 22 Min.
Column:
Mobile Phase:
Flow Rate:
Detector:
1. Chlordiazepoxide Degradant
2. Nitrazepam
3. Norchlordiazepoxide
4. Nordiazepam
3
2
5
4
1
Alltech ® Alltima HP C18 Amide, 5µm, 150 x 4.6mm
(Part No. 87733)
0.025M Potassium Phosphate Buffer, pH7.0:Methanol
(15:85)
1mL/min
UV at 254nm
Benzodiazepines and Metabolites
6
8691
5. Chlordiazepoxide
6. Chlordiazepoxide Degradant
7. Diazepam
7
Column: Alltech ® Alltima HP C8, 5µm, 150 x 4.6mm
(Part No. 87751)
Mobile Phase: A: 0.025M Potassium Phosphate Buffer, pH7.0
B: Methanol
Gradient: Time: 0 8 20
%B: 60 75 75
Flow Rate: 1.0mL/min
Detector: UV at 254nm
1
Universal High Speed Gradient
2
3
4
5
8957
1. Theophylline
2. Acetylsalicylic Acid
3. Propranolol
4. Nortriptyline
5. Trimipramine
0 2
4 6 Min.
Column: Alltech ® Platinum EPS C18, 3µm, 53 x 7mm Rocket
(Part No. 50573)
Mobile Phase: 0.05M Ammonium Acetate, pH5.5:Acetonitrile (65:35)
Flow Rate: 3.0mL/min
Detector: UV at 254nm
0 1 2 3 4 5 6 Min.
Column: Alltech ® Alltima C18, 3µm, 53 x 7mm Rocket
(Part No. 50605)
Mobile Phase: A: 0.1% Trifluoroacetic Acid in Water
B: 0.1% Trifluoroacetic Acid in Acetonitrile
Gradient: Time: 0 6
%B: 10 90
Flow Rate: 2.5mL/min
Detector: ELSD
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