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Water purification solutions - Prehrambeno-tehnološki fakultet Osijek

Water purification solutions - Prehrambeno-tehnološki fakultet Osijek

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<strong>Water</strong> contaminants:<br />

Summary of effects on HPLC performance<br />

Contaminants<br />

Effects on HPLC<br />

Improper check valve operation<br />

Particles<br />

Damage to pump and injector<br />

Plugging of frits and column<br />

Organics<br />

Ions<br />

Bacteria<br />

Ghost peaks<br />

Increased background noise<br />

Drifting baselines<br />

Some ions absorb in the UV range (e.g., NO 3- , NO 2- )<br />

With MS detectors: Na + , K + adduct peaks<br />

Plugging of frits and columns<br />

Ghost peaks

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