07.06.2015 Views

PASA Pipeline - Rice Genome Annotation Project

PASA Pipeline - Rice Genome Annotation Project

PASA Pipeline - Rice Genome Annotation Project

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Transcript Sequences<br />

<strong>PASA</strong> <strong>Pipeline</strong><br />

Seqclean<br />

Align to <strong>Genome</strong><br />

Cluster overlapping alignments<br />

<strong>PASA</strong> alignment assembly<br />

subCluster <strong>PASA</strong> assemblies<br />

Compare to annotation<br />

Update annotation<br />

<strong>Annotation</strong> Comparison<br />

FL-cDNAs and ESTs<br />

treated separately with<br />

different rules for<br />

incorporation<br />

<strong>Annotation</strong> Updates<br />

-exon modifications<br />

-alt splice isoform additions<br />

-gene merges<br />

-gene splits<br />

-new genes

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