CHAPTER 4: SCREENING FOR CERVICAL CANCER

PS 9: Collecting Samples for HPV DNA Testing 119PRACTICE SHEET 9: COLLECTING SAMPLES FOR HPV DNATESTINGPS 9For HPV DNA testing, secretions are collected from the cervix or vagina using a swabor small brush, and placed in a special liquid to be sent to the laboratory. There theycan be tested for HPV infection, which can stimulate changes in the cells covering thecervix. The test does not diagnose cervical precancer or cancer.The following materials and supplies are needed to collect samples for HPV testing:• soap and water for washing hands;• a light source to examine the cervix;• an examination table covered by clean paper or cloth;• a speculum, high-level disinfected (it need not be sterile);• disposable or high-level disinfected examination gloves;• small brush or soft swab;• small container with preservative solution;• recording form;• small container of warm water to lubricate and warm the speculum;• 0.5% chlorine solution for decontaminating instruments and gloves.Practice Sheet 9: Collecting Samples for HPV DNA TestingTAKING A SAMPLE FOR HPV TESTINGNote the following:• It is best not to take a sample from women who are actively menstruating. Slightbleeding is acceptable.• HPV testing, if available, is most useful when done in conjunction with acytological test, in women aged 35 years or older.Use Practice Sheet 4 to give counselling before doing any examination,test or procedure. Counselling steps specific to the HPV test are includedin the steps below.PS4Counselling