Control of Listeria in Processing Plants - bioMÃ©rieux

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Use of Sub-Typing as a ToolMolecular subtyping method• Generates a DNA fingerprint• Discrimination at the species, subspecies, or strain level• Allows for strain-to-strain comparisonUses in the food industry• Microbial source and spread tracking• Compare strains at different locations within a plant• Compare environmental isolate to finished product isolate• Niche identification
Use of Sub-Typing as a ToolPulsed-Field Gel Electrophoresis (PFGE)• Reproducibility• Ability to centralize and exchange typing data• Standardized and integrated (PulseNet, CDC)Repetitive element sequence-based PCR (rep-PCR)• Non-coding repetitive sequences found interspersed throughoutbacterial and fungal genomes• Rep elements found in all bacteria and fungi studied to date
Page 1 and 2: Control of Listeria in Processing P
Page 3 and 4: Significance of Listeria monocytoge
Page 5 and 6: Reasons for FSIS Recalls - 2011Ex M
Page 7 and 8: Food Recalls - Listeria monocytogen
Page 9 and 10: Critical Aspects of Preventing Post
Page 11 and 12: Microbial Mapping of the Plant Envi
Page 13 and 14: Sanitary ZonesZone 1Zone 2Zone 3Zon
Page 15 and 16: Growth NichesLocations harboring th
Page 17 and 18: Transfer Points vs NichesMany posit
Page 19: % PositiveSurveillance of Listeria
Page 23 and 24: SummaryMany recalls due to L. monoc
Page 25: Thank you!

Control of Listeria in Processing Plants - bioMÃ©rieux

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