Chlorophyll-A Analysis - Ohio Water Environment Association

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Spectrophotometric Technique• For water samples with high levels chlorophyll.Red LightRed Light(~665nm) into the(~665nm) into theSampleDetectorhttp://toolboxes.flexiblelearning.net.au/demosites/series10/10_03/6lt/6lt3/htm/6lt3_1_2e.htm
Equipment for Spec Method• Spectrophotometer –• Tissue Grinder• Centrifuge• Dark room• 47 mm glass fiber filters• Forceps• Filtration manifold• Filtration funnels andbases• 15 mL conical tubes• Volumetric or autopipettesw/tips• Aluminum foil• Freezer (-30 Celcius)
Page 1 and 2: Chlorophyll AnalysisDennis L. Hanse
Page 3 and 4: Quick Definitions• Chlorophyll is
Page 6 and 7: Lake Erie Has Algae• Is this Fish
Page 8 and 9: About The Algae: Green Algae• Fil
Page 10: About The Algae: Cyanobacteria• B
Page 13 and 14: The presence of N fixing Heterocyst
Page 15 and 16: Algal Blooms and Recreation•Non t
Page 17: Chlorophyll Detection Techniques•
Page 21 and 22: Overview of theSpectrophotometric M
Page 23 and 24: Overview of theSpectrophotometric M
Page 25 and 26: Equipment for LaboratoryFluorometri
Page 27: Summary• Chlorophyll A levels•

Chlorophyll-A Analysis - Ohio Water Environment Association

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