Chlorophyll-A Analysis - Ohio Water Environment Association

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Overview of theSpectrophotometric Method• In a darkened room• Centrifuge the sample to settle the filter and debrisand aspirate the supernatant• Or just pour the solution through a paper filter• Deliver a portion of the chlorophyll solution into acuvette and place the sample into thespectrophotometer
Overview of theSpectrophotometric Method• Read the absorbance at 665 nm• Compare this value to your standard curve andaccount for the dilution factors.• The method to manually calculate theconcentration is in US EPA method 150.1• Most labs use the Fluorometric method
Page 1 and 2: Chlorophyll AnalysisDennis L. Hanse
Page 3 and 4: Quick Definitions• Chlorophyll is
Page 6 and 7: Lake Erie Has Algae• Is this Fish
Page 8 and 9: About The Algae: Green Algae• Fil
Page 10: About The Algae: Cyanobacteria• B
Page 13 and 14: The presence of N fixing Heterocyst
Page 15 and 16: Algal Blooms and Recreation•Non t
Page 17 and 18: Chlorophyll Detection Techniques•
Page 19 and 20: Equipment for Spec Method• Spectr
Page 21: Overview of theSpectrophotometric M
Page 25 and 26: Equipment for LaboratoryFluorometri
Page 27: Summary• Chlorophyll A levels•

Chlorophyll-A Analysis - Ohio Water Environment Association

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