PaxGene Blood RNA Kit Handbook - Plastisch chirurgen

Figure 3 indicates the overall repeatability and reproducibility of the PAXgene BloodRNA System. Additional studies were conducted to show the influence of differentPAXgene Blood RNA kit lots and different operators on the reproducibility of RNA yieldand real time RT-PCR performance. As pooled blood samples instead of individualPAXgene tubes were used for these studies, the results do not reflect the systemrepeatability, including fluctuation between individual blood draws, but only therepeatability of the sample preparation (see Figure 4).Repeatability and Reproducibility of RNA Yield for Different Operators and PAXgeneBlood RNA Kit Lots Using Pooled Blood SamplesA14121086420Lot 1User 1User 2User 3Lot 2User 1User 2User 3Lot 3User 1User 2User 31 2 3 4 5 6 7 8 9 10Donor poolBCV of RNA yield (%)5040302010Lot 1User 1User 2User 3Lot 2User 1User 2User 3Lot 3User 1User 2User 301 2 3 4 5 6 7 8 9 10Donor poolFigure 4 Blood samples from 30 different donors were collected in PAXgene Blood RNA tubes (12 tubesper donor, 360 tubes in total). The contents of the tubes from 3 donors were pooled and subsequentlyre-aliquoted into 36 samples. These 36 samples per 3-donor-pool were processed by 3 different operators.Each operator used 3 different PAXgene Blood RNA Kit lots for the extraction and processed quadruplicatesamples from each of the 10 donor pools. A RNA yield and standard deviation for every operator–lotcombination. Quadruplicate blood samples from 10 donor pools were processed by 3 different operators(User 1, User 2, User 3) with each of 3 kit lots (Lot 1, Lot 2, Lot 3). The mean yields (columns) and standarddeviations (error bars) per quadruplicate sample from the same donor pool for different operator and differentkit lot are presented. B CV of RNA yield per donor pool for all operator–lot combinations (User 1, User 2,User 3; Lot 1, Lot 2, Lot 3) as calculated from the mean yield and standard deviation of the yield shown inFigure 4A.PAXgene Blood RNA Kit Handbook 06/2005 15