PaxGene Blood RNA Kit Handbook - Plastisch chirurgen

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Trademarks: PAXgene, PreAnalytiX (PreAnalytiX GmbH); BD Vacutainer ® , BD Hemogard, Safety-Lok (Becton, Dickinson and Company,Franklin Lakes, NJ, USA); Eppendorf ® (Eppendorf-Netheler-Hinz GmbH).The PCR process is covered by US Patents 4,683,195 and 4,683,202 and foreign equivalents issued to Hoffmann-La-Roche AG.Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law.© 2005 PreAnalytiX GmbH, all rights reserved.2PAXgene Blood RNA Kit Handbook 06/2005
ContentsExplanation of Symbols 4Kit Contents 5Storage Conditions 5Intended Use 6Product Use Limitations 6Quality Control 6Technical Assistance 6Safety Information 7Introduction 9Principle and procedure 9Sample collection and stabilization 9RNA concentration and purification 12Equipment and Reagents to Be Supplied by User 21ProtocolPurification of Total RNA from Human Whole Blood Collected into PAXgeneBlood RNA Tubes 22Troubleshooting Guide 27Appendix A: General Remarks on Handling RNA 29Appendix B: Quantification and Determination of Quality of Total RNA 30Appendix C: Handling PAXgene Blood RNA Tubes 32Ordering Information 33PAXgene Blood RNA Kit Handbook 06/2005 3
Page 1: Σ50 (cat. no. 762164)PAXgeneBlood
Page 6 and 7: Intended UseThe PAXgene Blood RNA K
Page 8 and 9: Buffer BR3Contains ethanol: flammab
Page 10 and 11: RNA Stability in Blood Samples at 1
Page 12 and 13: RNA concentration and purificationT
Page 14 and 15: A2420Reproducible and Repeatable RN
Page 16 and 17: Table 1A. Reproducibility within Ea
Page 18 and 19: Reproducibility of RT-PCR — betwe
Page 20 and 21: Table 2. Summary of RT-PCR Data fro
Page 22 and 23: Protocol: Purification of Total RNA
Page 24 and 25: ProtocolProcedure1. Centrifuge the
Page 26 and 27: Protocol15. Pipet 500 µl Buffer BR
Page 28 and 29: Comments and suggestionsLow A 260 /
Page 30 and 31: Appendix B: Quantification and Dete
Page 32 and 33: Appendix C: Handling PAXgene Blood
Page 34 and 35: PreAnalytiX products are distribute
Page 36: 1028434 06/2005

PaxGene Blood RNA Kit Handbook - Plastisch chirurgen

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