PaxGene Blood RNA Kit Handbook - Plastisch chirurgen

Reproducibility of RT-PCR — between Kit LotsA321∆∆C T0–1–2–31 2 3LotB321∆∆C T0–1–2–31 2 3LotFigure 6 RNA purified in the experiment described in Figure 4 was used for real-time RT-PCR. Relativetranscript levels of A FOS and B IL1B were determined by real-time, duplex RT-PCR using 18S rRNA as aninternal standard. The values for all samples are plotted, relative to the values for kit lot 1 (10 donor poolsx 3 users x 4 replicates = 120 data sets for each gene), with means (red lines) and standard deviations(black bars) or all samples shown. The dashed lines indicate the ± 3x total precision of the assays (FOS:2.34 C T ; IL1B, 1.93 C T ).PAXgene Blood RNA Kit Handbook 06/2005 19