PaxGene Blood RNA Kit Handbook - Plastisch chirurgen

Equipment and Reagents to Be Supplied by UserWhen working with chemicals, always wear a suitable lab coat, disposable gloves,and protective goggles. For more information, consult the appropriate material safetydata sheets (MSDSs), available from the product supplier.• PAXgene Blood RNA Tubes (cat. no. 762165)• Ethanol (96–100%, purity grade p.a.)• Pipets* (10 µl – 4 ml)• Sterile, aerosol-barrier, RNase-free pipet tips †• Graduated cylinder ‡• Variable-speed microcentrifuge* capable of attaining 1000–8000 x g, andequipped with a rotor for 2 ml microcentrifuge tubes• Centrifuge* capable of attaining 3000–5000 x g, and equipped with a swingoutrotor and buckets to hold PAXgene Blood RNA Tubes• Shaker–incubator* capable of incubating at 55°C and 65°C and shaking at ≥400rpm, not exceeding 1400 rpm (e.g., Eppendorf ® Thermomixer Compact, orequivalent)• Vortex mixer*• Disposable gloves* Ensure that instruments have been checked and calibrated regularly according to the manufacturer’srecommendations.†Ensure that you are familiar with the guidelines on handling RNA (Appendix A, page 29).‡For the addition of ethanol to Buffer BR4 concentrate.PAXgene Blood RNA Kit Handbook 06/2005 21