PaxGene Blood RNA Kit Handbook - Plastisch chirurgen

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Appendix C: Handling PAXgene Blood RNA TubesThe following recommendations from BD may be helpful when handling PAXgeneBlood RNA Tubes. See the PAXgene Blood RNA Tube Product Circular for moreinformation about PAXgene Blood RNA Tubes.Instructions for removal of BD Hemogard Closure1. Grasp the PAXgene Blood RNA Tube with one hand, placing the thumb under theBD Hemogard closure. (For added stability, place arm on solid surface.) With theother hand, twist the BD Hemogard closure while simultaneously pushing up withthe thumb of the other hand ONLY UNTIL THE TUBE STOPPER IS LOOSENED.2. Move thumb away before lifting closure. DO NOT use thumb to push closure offtube. Caution: If the tube contains blood, an exposure hazard exists. To helpprevent injury during closure removal, it is important that the thumb used to pushupward on the closure be removed from contact with the tube as soon as the BDHemogard closure is loosened.3. Lift closure off tube. In the unlikely event of the plastic shield separating from therubber stopper, DO NOT REASSEMBLE CLOSURE. Carefully remove rubber stopperfrom tube.Instructions for insertion of Secondary BD Hemogard Closure1. Replace closure over tube.2. Twist and push down firmly until stopper is fully reseated. Complete reinsertion ofthe stopper is necessary for the closure to remain securely on the tube duringhandling.32PAXgene Blood RNA Kit Handbook 06/2005
Ordering InformationProduct Contents Cat. no.PAXgene Blood RNA SystemProducts that can be ordered from QIAGENPAXgene Blood RNA Kit (50) 50 PAXgene Spin Columns, 762164*Processing Tubes,RNase-Free Reagents and Buffers.To be used in conjunction withthe PAXgene Blood RNA TubesProducts that can be ordered from BD and BD authorized distributors †PAXgene Blood RNA Tubes (100) 100 Blood Collection Tubes. 762165To be used in conjunction withthe PAXgene Blood RNA Kit (50)Blood Collection Set BD Vacutainer ® Safety-Lok 367281Blood Collection Set: 21G,0.75 inch needle, 12 inch tubingwith luer adapter; 50 per box,200 per caseBD Vacutainer One-Use Holder Case only for 13 mm and 36481516 mmdiameter; 1000/caseBD Vacutainer Plus Serum Tubes 13 x 75 mm 4.0 ml draw with 367812Red BD Hemogard closure andpaper label; 100/box,1000/case* USA only.†These blood collection accessories represent typical products that can be used withPAXgene Blood RNA Tubes. To find out more about these accessories, including how to order, visitwww.bd.com/vacutainer/products/venous .PAXgene Blood RNA Kit Handbook 06/2005 33
Page 1 and 2: Σ50 (cat. no. 762164)PAXgeneBlood
Page 3 and 4: ContentsExplanation of Symbols 4Kit
Page 6 and 7: Intended UseThe PAXgene Blood RNA K
Page 8 and 9: Buffer BR3Contains ethanol: flammab
Page 10 and 11: RNA Stability in Blood Samples at 1
Page 12 and 13: RNA concentration and purificationT
Page 14 and 15: A2420Reproducible and Repeatable RN
Page 16 and 17: Table 1A. Reproducibility within Ea
Page 18 and 19: Reproducibility of RT-PCR — betwe
Page 20 and 21: Table 2. Summary of RT-PCR Data fro
Page 22 and 23: Protocol: Purification of Total RNA
Page 24 and 25: ProtocolProcedure1. Centrifuge the
Page 26 and 27: Protocol15. Pipet 500 µl Buffer BR
Page 28 and 29: Comments and suggestionsLow A 260 /
Page 30 and 31: Appendix B: Quantification and Dete
Page 34 and 35: PreAnalytiX products are distribute
Page 36: 1028434 06/2005

PaxGene Blood RNA Kit Handbook - Plastisch chirurgen

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