PaxGene Blood RNA Kit Handbook - Plastisch chirurgen

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Table 1A. Reproducibility within Each Lot and within Each UserDonor pool 1 Donor pool 6 Donor pool 9 Donor pool 105.1 x 10 6 cells/ml 6.5 x 10 6 cells/ml 8.4 x 10 6 cells/ml 10.2 x 10 6 cells/mlMean SD CV Mean SD CV Mean SD CV Mean SD CVCombination of data yield (µg) (µg) (%) yield (µg) (µg) (%) yield (µg) (µg) (%) yield (µg) (µg) (%)Lot 1, user 1 8.03 0.42 5 9.55 0.99 10 7.52 0.41 6 7.96 0.49 6Lot 1, user 2 7.98 1.17 15 9.38 1.94 21 8.82 1.72 19 8.90 0.76 9Lot 1, user 3 7.87 0.45 6 10.71 0.65 6 10.14 1.46 14 10.22 1.29 13Lot 2, user 1 7.32 0.98 13 9.78 1.89 19 6.92 0.27 4 7.63 1.23 16Lot 2, user 2 6.09 1.04 17 9.82 2.83 29 7.20 0.71 10 7.00 0.56 8Lot 2, user 3 6.87 0.31 4 10.37 0.74 7 9.14 1.52 17 11.56 1.21 10Lot 3, user 1 7.04 0.90 13 8.96 0.68 8 8.18 0.76 9 7.85 0.82 10Lot 3, user 2 6.98 1.22 17 7.73 0.97 13 6.41 0.88 14 8.88 2.17 24Lot 3, user 3 8.78 0.89 10 10.59 1.94 18 10.78 0.56 5 10.88 0.37 3Table 1B. Reproducibility within Each User and between All LotsDonor pool 1 Donor pool 6 Donor pool 9 Donor pool 105.1 x 10 6 cells/ml 6.5 x 10 6 cells/ml 8.4 x 10 6 cells/ml 10.2 x 10 6 cells/mlMean SD CV Mean SD CV Mean SD CV Mean SD CVCombination of data yield (µg) (µg) (%) yield (µg) (µg) (%) yield (µg) (µg) (%) yield (µg) (µg) (%)User 1, all lots 7.46 0.85 11 9.43 1.22 13 7.54 0.72 10 7.81 0.82 11User 2, all lots 7.02 1.31 19 8.98 2.09 23 7.48 1.50 20 8.26 1.54 19User 3, all lots 7.84 0.98 13 10.56 1.15 11 10.02 1.34 13 10.89 1.10 1016PAXgene Blood RNA Kit Handbook 06/2005
Table 1C. Reproducibility within Each Lot and between All UsersDonor pool 1 Donor pool 6 Donor pool 9 Donor pool 105.1 x 10 6 cells/ml 6.5 x 10 6 cells/ml 8.4 x 10 6 cells/ml 10.2 x 10 6 cells/mlMean SD CV Mean SD CV Mean SD CV Mean SD CVCombination of data yield (µg) (µg) (%) yield (µg) (µg) (%) yield (µg) (µg) (%) yield (µg) (µg) (%)Lot 1, all users 7.96 0.69 9 9.88 1.34 14 8.83 1.63 19 9.02 1.27 14Lot 2, all users 6.76 0.93 14 9.99 1.84 18 7.75 1.36 18 8.73 2.31 26Lot 3, all users 7.60 1.27 17 9.09 1.71 19 8.46 1.99 24 9.20 1.80 20Table 1D. Reproducibility between All Lots and All UsersDonor pool 1 Donor pool 6 Donor pool 9 Donor pool 105.1 x 10 6 cell/ml 6.5 x 10 6 cell/ml 8.4 x 10 6 cell/ml 10.2 x 10 6 cell/mlMean SD CV Mean SD CV Mean SD CV Mean SD CVCombination of data yield (µg) (mg) (%) yield (µg) (µg) (%) yield (µg) (µg) (%) yield (µg) (µg) (%)All lots and all users 7.44 1.09 15 9.66 1.65 17 8.35 1.70 20 8.99 1.80 20Detailed analysis of 4 representative donor pools. The pools were selected according to the white blood cell count and reflect the upper, medium, and the lowervalues of the normal range of white blood cell counts (4.8 x 10 6 – 1.1 x 10 7 leukocytes/ml). The white blood cell count represents the mean value of the 3 whiteblood cell counts from the 3 donors per donor pool.PAXgene Blood RNA Kit Handbook 06/2005 17
Page 1 and 2: Σ50 (cat. no. 762164)PAXgeneBlood
Page 3 and 4: ContentsExplanation of Symbols 4Kit
Page 6 and 7: Intended UseThe PAXgene Blood RNA K
Page 8 and 9: Buffer BR3Contains ethanol: flammab
Page 10 and 11: RNA Stability in Blood Samples at 1
Page 12 and 13: RNA concentration and purificationT
Page 14 and 15: A2420Reproducible and Repeatable RN
Page 18 and 19: Reproducibility of RT-PCR — betwe
Page 20 and 21: Table 2. Summary of RT-PCR Data fro
Page 22 and 23: Protocol: Purification of Total RNA
Page 24 and 25: ProtocolProcedure1. Centrifuge the
Page 26 and 27: Protocol15. Pipet 500 µl Buffer BR
Page 28 and 29: Comments and suggestionsLow A 260 /
Page 30 and 31: Appendix B: Quantification and Dete
Page 32 and 33: Appendix C: Handling PAXgene Blood
Page 34 and 35: PreAnalytiX products are distribute
Page 36: 1028434 06/2005

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