NR <strong>51</strong> 2010AN TRO PO MO TO RY KATHE INFLUENCE OF PHYSICAL EFFORT IN WARMCONDITIONS ON CHANGING THE WHITE BLOODCELLS NUMBER IN TRAININGAND NON-TRAINING MENWPŁYW WYSIŁKU FIZYCZNEGOW PODWYŻSZONEJ TEMPERATURZE OTOCZENIANA ZMIANY W OBRAZIE BIAŁOKRWINKOWYMU TRENUJĄCYCH I NIETRENUJĄCYCH MĘŻCZYZNWanda Pilch*, Marta Szarek**, Dorota Gryka-Nowaczyk**,Michał Kaczmarek***, Wacław Mirek******* Dr. habil., Physiology and Biochemistry Unit, Institute of Human Physiology, University School of Physical Education,Cracow, Poland**** MSc, Physiology and Biochemistry Unit, Institute of Human Physiology, University School of Physical Education,Cracow, Poland**** MSc, Doctoral Studies, University School of Physical Education, Cracow, Poland**** PhD, Theory and Methodology of Track and Field Athletics Department, University School of Physical Education,Cracow, PolandKey words: thermoregulation, athletes, white blood cells, exercise in the heatSłowa kluczowe: termoregulacja, lekkoatleci, leukocyty, wysiłek w podwyższonejtemperaturzeSUMMARY • STRESZCZENIEAim of the work. The purpose of this study was to determine the influence of warm and humid conditionson changing the number of white blood cells during submaximal effort in training and non-training healthymen group.Material and methods. The study involved 2 groups of 10 healthy men. The first group consisted of 10medium- and long-distance runners who had similar aerobic working capacity as well as similar length of training(5 ± 1.2 years). The second – control group – consisted of 10 healthy non-training men. The tests wereperformed on a cycloergometer with individually estimated work load of 53 ± 2% VO 2max, in the test-roomin which the temperature of 33 °C ± 1 °C and humidity of 70% was maintained. The effort was continued tothe point when the core temperature of subjects measured per rectum increased by 1.2 °C. Before and afterthe test the body weight was measured as well as the core temperature of the subjects was monitored duringthe exercise. In the blood samples collected before and after the test the total protein level and hematologicalindexes were determined.Results and conclusions. This study showed that the physical effort in warm and humid conditions resultsin decrease of the body mass and the plasma volume contraction in both groups. However, the work done bythe athletes was found to be longer and harder compared with the control group. Intense sweating during theexercise caused a higher decrease of the plasma volume in the training group than in non-training group. Therise in the number of lymphocytes, neutrophiles and basophiles accompanied by the increase of the total whiteblood cells was observed in the regularly training group of men, whereas in the control group only the numberof neutrophiles and eosinophiles increased significantly. The results of the study show that after effort leuco-– 61 –
Wanda Pilch, Marta Szarek, Dorota Gryka-Nowaczyk, Michał Kaczmarekcytosis was remarkably higher in the athletes in comparison with the non-training group. Moreover, the workdone by the athletes which caused the increase in the core temperature by 1.2 °C was significantly harder.Cel pracy. Określenie wpływu wysiłku submaksymalnego w otoczeniu o podwyższonej temperaturze i wilgotnościna ilościowe zmiany leukocytów u osób trenujących i nietrenujących.Materiał i metody. Badaniami objęto 10 lekkoatletów uprawiających biegi długo- i średniodystansowe,posiadających podobny poziom wydolności aerobowej i podobny staż treningowy (5 ± 1,2 lat) oraz losowo zakwalifikowanychdo grupy kontrolnej mężczyzn nieuprawiających wyczynowo sportu. Badani wykonywali wysiłekna cykloergometrze z indywidualnie wyznaczonym obciążeniem 53 ± 2% VO 2max w komorze termoklimatycznejw temperaturze 33 °C ± 1 °C oraz wilgotności względnej 70%. Wysiłek był kontynuowany do momentu, w którymtemperatura mierzona per rectum podwyższyła się o 1,2 °C. W trakcie badań oznaczano zmiany masy ciałai temperatury wewnętrznej badanych oraz we krwi pobieranej przed i po wysiłku, oznaczano białko całkowite orazwskaźniki hematologiczne.Wyniki i wnioski. Przeprowadzony eksperyment wykazał, że wysiłek w podwyższonej temperaturze i wilgotnościpowietrza wpłynął na obniżenie masy ciała i objętości osocza badanych osób. Lekkoatleci wykonali dłuższąi większą pracę niż osoby nietrenujące. Intensywne pocenie w czasie wysiłku spowodowało większy ubytekobjętości osocza u lekkoatletów w porównaniu do osób nietrenujących. W obrazie białokrwinkowym biegaczy powysiłku wystąpił wzrost całkowitej liczby krwinek białych, limfocytów, neutrofilów oraz bazofilów. Natomiast wekrwi osób nietrenujących zaobserwowano wzrost liczby neutrofilów i eozynofilów. Znacznie większa powysiłkowaleukocytoza wystąpiła u lekkoatletów, którzy wykonali znacznie większą pracę celem podniesienia temperaturywewnętrznej o 1,2 °C w porównaniu z mężczyznami nietrenującymi.IntroductionPhysical activity in high temperatures requires the intensificationof the thermoregulatory mechanisms.Under such conditions, heat both penetrates into thebody from a hot environment and is generated duringan intense physical work. Excess heat must be eliminatedfrom the body in order to prevent hyperthermia.Under these conditions, the most effective way of heatelimination is the binding of thermal energy during theevaporation of sweat. Undertaking physical activity inconditions of high humidity puts additional strain on thebody and reduces exercise capabilities. Steam transfersmore heat to the body in high temperature environmentthan dry air. High humidity hinders the body’scooling mechanism, i.e. sweating. Consequently, bodytemperature rises and the load of the respiratory systemand the circulatory system increases. Under conditionsof high temperature and humidity, the mechanism ofheat elimination through sweating becomes less effective,which leads to dehydration and increase in internalbody temperature. Under such conditions, the lack offluid intake causes the inability to continue exercise.Physical activity in hot humid environment causesthe loss of body fluids through sweating. Sweat ratemay reach 2–3 litres per hour. Water penetrates fromthe intercellular space to sweat, which reduces the volumeof plasma and circulating blood [1].Numerous studies prove that physical activitycauses changes in the immune system. The increasein leukocyte number after physical effort is the mostthoroughly studied effect [2, 3]. This phenomenon iscalled myogenic leukocytosis [4].Acute and immediate leukocytosis escalates indirect proportion to intensity and duration of physicalactivity and in inverse proportion to the body’s fitnesslevel [5, 6, 7]. Neutrocytes play the main role in theincrease of leukocyte number during and after physicaleffort. Lymphocytes have less influence, thoughthe number of monocytes increases, too. After approx2 hours of restitution, the increased number of neutrocytesdoes not change, and the number of lymphocytesdecreases [8].It is assumed that the increase in leukocyte numberinduced by physical effort may result from leukocyte redistribution.The increase in stroke volume and bloodflow velocity may cause release of marginal cells (atrest, for example in the lungs, in the liver, and in thespleen) into the circulation [9].Researchers find it difficult to explain the influenceof physical activity on quantitative changes in leukocytepopulations and mechanisms responsible for the abovementioned changes. Research results are not unequivocal,which may be connected with the complexity ofthe immunological system and various factors influencingits functioning [10].The available literature lacks the data concerningthe combined influence of high temperature, humidityand effort on the size of changes of the picture ofleukocytes. The majority of previous research studies– 62 –
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