Agilent capillary electrophoresis system

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CE solutions – for discovery,development and quality controlof drugsIn today’s demanding pharmaceutical industry, fast and robust separation methods areneeded in order to reduce the time to market for new drugs. Capillary electrophoresis,the powerful instrumental approach to electrophoresis, responds to these needs in allstages from drug discovery through quality control. CE offers fast separations withorthogonal selectivities to chromatographic techniques. In addition, the integration ofVitaminsCE into the US Pharmacopeia as a standard physical test—initiated in 1996, ensures itsacceptance worldwide.Agilent Technologies has made a long-term commitment to the pharmaceutical industry.Changing regulatory requirements are monitored all over the world to ensure you get theright product features and services—a large number of applications and solution kitshave already been developed as part of this process.OligonucleotidesAbsorbance [mAU]60ChecklistMethod:þ Fast separationþ Robustþ Low cost per analysisþ Regulatory compliance toolsþ Simultaneous quantification of maincomponent and trace contaminantsHardware:þ Agilent high sensitivity detection cellþ Replenishment for unattended operationþ CEC capabilityþ Single vendor CE-MS solutionSoftware:þ Multitechnique softwareþ Self-explanatory user interfaceþ Direct access to instrument controlþ Enhanced integrator for CE peaksþ Mobility calculation for improvedreproducibilityþ Integrated CE-MS controlþ Data accessibility for the next millennium50403020100without organicadditivewith organicadditive5 10 15 20 25 30 35OligonucleotidesThe analysis of oligonucleotides orantisense oligonucleotide therapeuticsby capillary electrophoresis is fast,reproducible and highly automated.This makes it ideally suitable for qualitycontrol applications in oligonucleotidecore facilities, as well as in many phasesof bio pharmaceutical drug development.ProteinsTime [min]
Absorbance[mAU]PeptidesDouble stranded DNA and PCR productsCapillary electrophoresis provides aconvenient method to determine thesize of double-stranded DNA and PCRproducts. The Agilent ChemStationautomatically generates a Ferguson plotfor size determination.864202.72.62.52.42.32.22.1-36 bp-51 bp-65 bp-75 bpLog BP-126 bp0.08 0.09 1/MT-179 bp-222 bp-350 bp-396 bp-480 bp-517 bp-676 bp-1,198 bp-1,605 bp8 9 10 11 12 13 14 15-2,654 bpTime [min]DNA“Regulatory authorities are increasingly requesting that the pharmaceutical industryassess the enantiomeric purity of their chiral building blocks, intermediates and finaldrug substances. This, coupled with the ever increasingdemands imposed on the analyst to reduce methodAbsorbance [mAU]development times, makes chiral CE a very attractivetechnique. It offers unprecedented chiral discrimination through its high resolving power, chiral selector500versatility and rapid automated method development.”400S-formDr. Melvin Euerby(Astra Charnwood, UK)300R-form2000.05 % area/area100Chirals00 10 20 30Time [min]Chiral drug compoundWhen used with the high sensitivitydetection cell the simultaneous quantificationof the main component and traceimpurities below 0.1 % is possible withoutsample overloading.Glycoproteins
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Agilent capillary electrophoresis system

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