Mould growth on building materials - Statens Byggeforskningsinstitut
Mould growth on building materials - Statens Byggeforskningsinstitut
Mould growth on building materials - Statens Byggeforskningsinstitut
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3 OVERVIEW OF EXPERIMENTAL WORK<br />
The details of the experiments performed during this study can be found in the papers 1-13.<br />
However results of a number of small experiments not included in the papers can be found in<br />
the discussi<strong>on</strong> part and identificati<strong>on</strong> of comp<strong>on</strong>ents initially reported as unknown.<br />
Time<br />
Growth tests of a<br />
mixture of 8<br />
species <strong>on</strong><br />
different<br />
<strong>materials</strong> under<br />
different humidity<br />
and temperature<br />
c<strong>on</strong>diti<strong>on</strong>s<br />
Paper #10<br />
A. versicolor, S. chartarum and Trichoderma<br />
spp. grown <strong>on</strong> <strong>building</strong> <strong>materials</strong>.<br />
Development of extracti<strong>on</strong> methods for the<br />
mycotoxins prior to HPLC analysis.<br />
Paper #2<br />
Chaetomium spp., Alternaria spp., Ulocladium spp., P.<br />
chrysogenum, P. pol<strong>on</strong>icum, P. brevicompactum, A. ustus and<br />
A. niger grown <strong>on</strong> <strong>building</strong> <strong>materials</strong>. Naturally infested material<br />
also collected, including some with A. versicolor<br />
Paper #3<br />
Development of<br />
quantitative EI +<br />
GC-MS/MS<br />
methods for<br />
ergosterol<br />
Paper #5<br />
Genetic, toxicity<br />
and metabolite<br />
profiles of<br />
Stachybotrys<br />
isolates<br />
Paper #7<br />
<str<strong>on</strong>g>Mould</str<strong>on</strong>g> <str<strong>on</strong>g>growth</str<strong>on</strong>g><br />
and metabolism<br />
<strong>on</strong> <strong>building</strong><br />
<strong>materials</strong> under<br />
c<strong>on</strong>stant and<br />
transient<br />
humidities.<br />
Performed at<br />
TNO, Paper #13<br />
Figure 4. Overview of experimental work.<br />
Development of fast solid<br />
phase extracti<strong>on</strong> and<br />
HPLC method for<br />
macrocyclic<br />
trichothecenes from S.<br />
chartarum. Paper #6<br />
Comparis<strong>on</strong> of cytotoxicity<br />
and inflammatory<br />
resp<strong>on</strong>ses and metabolite<br />
profiles of Stachybotrys<br />
isolates. Paper #9<br />
Double blinded exposure<br />
of humans to spores of P.<br />
chrysogenum and T.<br />
harzianum.<br />
To be published<br />
Method comparis<strong>on</strong> of<br />
ergosterol and<br />
Mycometer test<br />
Paper #12<br />
Page 30<br />
Collecti<strong>on</strong> of S. chartarum infested<br />
<strong>materials</strong>. Development of solid<br />
phase extracti<strong>on</strong> and GC-MS NICI<br />
method for trichothecenes<br />
Paper #1<br />
Development of fast<br />
GC-MS/MS methods<br />
for Stachybotrys and<br />
Fusarium<br />
trichothecenes.<br />
Paper #8<br />
Comparis<strong>on</strong> of<br />
MVOC's and satratoxin<br />
producti<strong>on</strong><br />
Paper #11<br />
Analysing air<br />
samples from<br />
Stachybotrys<br />
infested houses<br />
and isolates from<br />
the Belgian IPH<br />
case home<br />
To be published