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Mould growth on building materials - Statens Byggeforskningsinstitut

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3 OVERVIEW OF EXPERIMENTAL WORK<br />

The details of the experiments performed during this study can be found in the papers 1-13.<br />

However results of a number of small experiments not included in the papers can be found in<br />

the discussi<strong>on</strong> part and identificati<strong>on</strong> of comp<strong>on</strong>ents initially reported as unknown.<br />

Time<br />

Growth tests of a<br />

mixture of 8<br />

species <strong>on</strong><br />

different<br />

<strong>materials</strong> under<br />

different humidity<br />

and temperature<br />

c<strong>on</strong>diti<strong>on</strong>s<br />

Paper #10<br />

A. versicolor, S. chartarum and Trichoderma<br />

spp. grown <strong>on</strong> <strong>building</strong> <strong>materials</strong>.<br />

Development of extracti<strong>on</strong> methods for the<br />

mycotoxins prior to HPLC analysis.<br />

Paper #2<br />

Chaetomium spp., Alternaria spp., Ulocladium spp., P.<br />

chrysogenum, P. pol<strong>on</strong>icum, P. brevicompactum, A. ustus and<br />

A. niger grown <strong>on</strong> <strong>building</strong> <strong>materials</strong>. Naturally infested material<br />

also collected, including some with A. versicolor<br />

Paper #3<br />

Development of<br />

quantitative EI +<br />

GC-MS/MS<br />

methods for<br />

ergosterol<br />

Paper #5<br />

Genetic, toxicity<br />

and metabolite<br />

profiles of<br />

Stachybotrys<br />

isolates<br />

Paper #7<br />

<str<strong>on</strong>g>Mould</str<strong>on</strong>g> <str<strong>on</strong>g>growth</str<strong>on</strong>g><br />

and metabolism<br />

<strong>on</strong> <strong>building</strong><br />

<strong>materials</strong> under<br />

c<strong>on</strong>stant and<br />

transient<br />

humidities.<br />

Performed at<br />

TNO, Paper #13<br />

Figure 4. Overview of experimental work.<br />

Development of fast solid<br />

phase extracti<strong>on</strong> and<br />

HPLC method for<br />

macrocyclic<br />

trichothecenes from S.<br />

chartarum. Paper #6<br />

Comparis<strong>on</strong> of cytotoxicity<br />

and inflammatory<br />

resp<strong>on</strong>ses and metabolite<br />

profiles of Stachybotrys<br />

isolates. Paper #9<br />

Double blinded exposure<br />

of humans to spores of P.<br />

chrysogenum and T.<br />

harzianum.<br />

To be published<br />

Method comparis<strong>on</strong> of<br />

ergosterol and<br />

Mycometer test<br />

Paper #12<br />

Page 30<br />

Collecti<strong>on</strong> of S. chartarum infested<br />

<strong>materials</strong>. Development of solid<br />

phase extracti<strong>on</strong> and GC-MS NICI<br />

method for trichothecenes<br />

Paper #1<br />

Development of fast<br />

GC-MS/MS methods<br />

for Stachybotrys and<br />

Fusarium<br />

trichothecenes.<br />

Paper #8<br />

Comparis<strong>on</strong> of<br />

MVOC's and satratoxin<br />

producti<strong>on</strong><br />

Paper #11<br />

Analysing air<br />

samples from<br />

Stachybotrys<br />

infested houses<br />

and isolates from<br />

the Belgian IPH<br />

case home<br />

To be published

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