PixFRET, an ImageJ plug-in for FRET calculation which can ...

More documents

Recommendations

Info

BF CFP /CFP CFP0.70.60.50.40.30.20.100 50 100 150 200 250F CFP /CFP CFP0.70.60.50.40.30.20.1CFP CFP00 50 100 150 200 250CFP CFPFeige et al. - Fig. 2BCFP CFP PMT2 : 530V / Laser : 70%F CFP PMT2 : 530V / Laser : 70%CFP CFP PMT2 : 630V / Laser : 25%F CFP PMT2 : 630V / Laser : 25%F CFP /CFP CFP0.70.60.50.40.30.20.100 50 100 150 200 250F CFP /CFP CFP0.70.60.50.40.30.20.1CFP CFP0CFP CFP PMT2 : 730V / Laser : 15%F CFP PMT2 : 730V / Laser : 15%0 50 100 150 200 250CFP CFPCFP CFP PMT2 : 830V / Laser : 10%F CFP PMT2 : 830V / Laser : 10%
Feige et al. - Fig. 3ABBT CFP = c CFP x CFP CFP + d CFPBT CFP = e CFP x exp(CFP CFP x f CFP ) + g CFPc CFP = 0.0868d CFP = 33.33R 2 = 0.68e CFP = 0.0076f CFP = 0.0158g CFP = 0.37R 2 = 0.760.70.7SBT ratio = F CFP /CFP CFP0.60.50.40.30.20.1Average SBTratio0.60.50.40.30.20.1Average SBTratio00 50 100 150 200 250CFP CFP00 50 100 150 200 250CFP CFP
Page 1 and 2: PixFRET, an ImageJ plug-in for FRET
Page 3 and 4: IntroductionFluorescence Resonance
Page 5 and 6: Material and MethodsPlasmid constru
Page 7 and 8: available at: http://rsb.info.nih.g
Page 9 and 10: SBT was analyzed on a Zeiss LSM510
Page 11 and 12: controls as, under these experiment
Page 13 and 14: were introduced to discard points i
Page 15 and 16: DiscussionFRET is a technique whose
Page 17 and 18: In conclusion, we uncovered the bia
Page 19 and 20: AcknowledgementsWe thank Dr Claude
Page 21 and 22: ECFP and RXRα-EYFP. Fluorescence i
Page 23: AF CFP /CFP CFP0.70.60.50.40.30.20.
Page 27 and 28: ABFeige et al. - Fig. 5

PixFRET, an ImageJ plug-in for FRET calculation which can ...

Create successful ePaper yourself

Delete template?

Save as template?