Core facilities at EMBL â an overview - Molmedrex Project

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EMBL Solexas• ~180 million reads per a flow-cell (8 lanes)• >7 Gbp per single read flow-cell (36 bases)• >14 Gbp per PE flow-cell (36 bases)• >2 Gbp/day (capillary sequencer max. 320 kb/day)• 2,5 day single read run, 5 day PE run on Solexa (36 bases)• Supported reading length: 75 bases, 100 coming• Sequencing libraries 200-500 bp, 3-5 kb “insert” length(mate-pair), multiplexing• Full sample processing (library preparation, clustering,sequencing): ~950 €/sample/lane (single-read, 36 bases)
Illumina-Solexa workflow1) Library preparationSingle readPaired-end read36-100 basesBarcoding2) Cluster generation ona flow cell3) Sequencing & imaging4) Data processing & analysis
Page 1 and 2: Core facilities at EMBL -an overvie
Page 3: Research & Core Facilities at EMBLP
Page 6 and 7: Core Facilities at EMBL• Advanced
Page 8 and 9: Unlocking the genomeCelniker et al.
Page 10 and 11: GeneCore ServicesLiquid handling ro
Page 12 and 13: GeneCore ServicesComplete support &
Page 14 and 15: Regulation of ERα responsive genes
Page 18 and 19: Cluster generationPrepare DNAfragme
Page 20 and 21: Applications at EMBL• Whole-genom
Page 22 and 23: Challenges• ChIP-Seq and RNA-Seq
Page 25 and 26: AcknowledgementRichard CarmoucheJü

Core facilities at EMBL â an overview - Molmedrex Project

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Core facilities at EMBL â an overview - Molmedrex Project