Core facilities at EMBL â an overview - Molmedrex Project

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Applications at EMBL• Whole-genome analyses of transcriptome,miRNome & occupancy profiling• Identification of DNA and RNA protein binding sites(ChIP-Seq, CLIP-Seq)• Identification of methylation patterns (Methyl-Seq)• Identification of small ncRNA (miRNA) sequences• Digital gene expression profiling (RNA-Seq)• Whole genome sequencing• De novo sequencing and re-sequencing (pairedendedreads)• Structural variation of genomes – CNV, SNP, in/del
Data analysis –sequence census approach3 types of information:• Sequence polymorphism in the template• Quantitative measure (abundance of reads)• Covered regions reveal the internalstructure of the template (exon/introns, NAproteinbinding sites)Morozova & Marra, Genomics (2008)
Page 1 and 2: Core facilities at EMBL -an overvie
Page 3: Research & Core Facilities at EMBLP
Page 6 and 7: Core Facilities at EMBL• Advanced
Page 8 and 9: Unlocking the genomeCelniker et al.
Page 10 and 11: GeneCore ServicesLiquid handling ro
Page 12 and 13: GeneCore ServicesComplete support &
Page 14 and 15: Regulation of ERα responsive genes
Page 16 and 17: EMBL Solexas• ~180 million reads
Page 18 and 19: Cluster generationPrepare DNAfragme
Page 22 and 23: Challenges• ChIP-Seq and RNA-Seq
Page 25 and 26: AcknowledgementRichard CarmoucheJü

Core facilities at EMBL â an overview - Molmedrex Project

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Core facilities at EMBL â an overview - Molmedrex Project