Core facilities at EMBL â an overview - Molmedrex Project
Core facilities at EMBL â an overview - Molmedrex Project
Core facilities at EMBL â an overview - Molmedrex Project
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Challenges• ChIP-Seq <strong>an</strong>d RNA-Seq still requires rel<strong>at</strong>ively high startingamount of IP or total RNA• Output is not yet qu<strong>an</strong>tit<strong>at</strong>ive enough~10 million reads to qu<strong>an</strong>tify ~ 80 %of tr<strong>an</strong>scripts, ~200 million reads to qu<strong>an</strong>tifysplicing levels for ~80 % of tr<strong>an</strong>scriptsBlencowe, Genes & Development (2009)• Tools for qu<strong>an</strong>tific<strong>at</strong>ion of sequencing library to determinenumber of fragments th<strong>at</strong> c<strong>an</strong> gener<strong>at</strong>e clusters suboptimal• Reduction of base composition bias in produced d<strong>at</strong>a• D<strong>at</strong>a <strong>an</strong>alysis
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