Standard Operating Procedure - Ultrafiltration - Department of ...

Note: there may be residual fluid in the system’s supply lines, modules, etc.A bucket or other container will be needed to prevent spills during operation.Before configuring the Model L, ensure that the unit is safe by:• Verify that the pump power cord [18] is disconnected.• Confirm that no cooling water is flowing through the unit by verifying that heatexchanger inlet valve [5] is closed and t6he main water supply valve is closed.• Confirm that system is depressurized by verifying that pressure control valve [12] is fullyopen and that both pressure gauges [14, 15] read no system pressure.• Drain system by opening reservoir drain valve [21] (with appropriate bucket orcontainer present).M20 Lab ModuleIf a flat sheet membrane is to be used the Model L should be configured to utilize theM20 Lab Module [8] by following these steps:1. Connect the feed line [3] to the M20 feed inlet [9].2. Loosen butterfly clamp [1] at end of feed line and disconnect feed line.3. Inspect supply line O-ring [28]. Discard and replace if any defects (cuts, perforations,brittleness, etc.) are found.4. Lubricate O-ring with vacuum grease, insert O-ring between end of feed line and M20feed inlet, and secure connection with butterfly clamp.5. Connect retentate line [22] to M20 retentate outlet [10]6. Follow same procedure as in step two above (connecting feed line)7. Build filter stack according to instructions outlined in this SOP.34