References127. Park S, Han SS, Park CH, Hahm ER, Lee SJ, Park HK, Lee SH, Kim WS, Jung CW, ParkK, Riordan HD, Kimler BF, Kim K, Lee JH. L-ascorbic acid <strong>in</strong>duces apoptosis <strong>in</strong> acutemyeloid leukemia cells via hydrogen peroxide-mediated mechanisms. Int J Biochem Cell Biol.2004;36:2180-2195128. Iwasaka K, Koyama N, Nogaki A, Maruyama S, Tamura A, Takano H, Takahama M,Kochi M, Satoh K, Sakagami H. Role <strong>of</strong> hydrogen peroxide <strong>in</strong> cytotoxicity <strong>in</strong>duction byascorbates and other redox compounds. Anticancer Res. 1998;18:4333-4337129. Gechev TS, Hille J. Hydrogen peroxide as a signal controll<strong>in</strong>g plant programmed celldeath. J Cell Biol. 2005;168:17-20130. Clement MV, Ponton A, Pervaiz S. Apoptosis <strong>in</strong>duced by hydrogen peroxide is mediatedby decreased superoxide anion concentration and reduction <strong>of</strong> <strong>in</strong>tracellular milieu. FEBSLett. 1998;440:13-18131. Hampton MB, Orrenius S. Dual regulation <strong>of</strong> caspase <strong>activity</strong> by hydrogen peroxide:Implications for apoptosis. FEBS Lett. 1997;414:552-556132. Gonzalez-Flecha B, Demple B. Homeostatic regulation <strong>of</strong> <strong>in</strong>tracellular hydrogen peroxideconcentration <strong>in</strong> aerobically grow<strong>in</strong>g Escherichia coli. J Bacteriol. 1997;179:382-388133. Fenton HJH. On a new reaction <strong>of</strong> tartaric acid. Chem News. 1876;33:190134. Halliwell B, Gutteridge JM. Biologically relevant metal ion-dependent hydroxyl radicalgeneration. An update. FEBS Lett. 1992;307:108-112135. Wardman P, Candeias LP. Fenton chemistry: An <strong>in</strong>troduction. Radiat Res. 1996;145:523-531136. Burkitt MJ, Gilbert BC. Model studies <strong>of</strong> the iron-catalysed Haber-Weiss cycle and theascorbate-driven Fenton reaction. Free Radic Res Commun. 1990;10:265-280137. Prabhu HR, Krishnamurthy S. Ascorbate-dependent formation <strong>of</strong> hydroxyl radicals <strong>in</strong> thepresence <strong>of</strong> iron chelates. Indian J Biochem Biophys. 1993;30:289-292138. L<strong>in</strong>dqvist C, Nordstrom T. Generation <strong>of</strong> hydroxyl radicals by the antiviral compoundphosphon<strong>of</strong>ormic acid (foscarnet). Pharmacol Toxicol. 2001;89:49-55139. Gutteridge JM. Superoxide-dependent formation <strong>of</strong> hydroxyl radicals from ferriccomplexesand hydrogen peroxide: An evaluation <strong>of</strong> fourteen iron chelators. Free Radic ResCommun. 1990;9:119-125140. Haber F, Weiss J. The catalytic decomposition <strong>of</strong> hydrogen peroxide by iron salts. <strong>Pro</strong>c RoySoc. 1934;A147:332-351141. Kehrer JP. The Haber-Weiss reaction and mechanisms <strong>of</strong> toxicity. Toxicology. 2000;149:43-5057
References142. Rosen GM, Rauckman EJ. Sp<strong>in</strong> trapp<strong>in</strong>g <strong>of</strong> superoxide and hydroxyl radicals. MethodsEnzymol. 1984;105:198-209143. Halliwell B, Grootveld M, Gutteridge JM. Methods for the measurement <strong>of</strong> hydroxylradicals <strong>in</strong> biomedical systems: Deoxyribose degradation and aromatic hydroxylation.Methods Biochem Anal. 1988;33:59-90144. Richmond R, Halliwell B, Chauhan J, Darbre A. Superoxide-dependent formation <strong>of</strong>hydroxyl radicals: Detection <strong>of</strong> hydroxyl radicals by the hydroxylation <strong>of</strong> aromaticcompounds. Anal. Biochem. 1981;118:328-335145. S<strong>in</strong>gh S, Hider RC. Colorimetric detection <strong>of</strong> the hydroxyl radical: Comparison <strong>of</strong> thehydroxyl-radical-generat<strong>in</strong>g ability <strong>of</strong> various iron complexes. Anal. Biochem. 1988;171:47-54146. Halliwell B, Gutteridge JM, Aruoma OI. The deoxyribose method: A simple "test-tube"assay for determ<strong>in</strong>ation <strong>of</strong> rate constants for reactions <strong>of</strong> hydroxyl radicals. Anal. Biochem.1987;165:215-219147. Rowley DA, Halliwell B. Superoxide-dependent and ascorbate-dependent formation <strong>of</strong>hydroxyl radicals <strong>in</strong> the presence <strong>of</strong> copper salts: A physiologically significant reaction?Arch Biochem Biophys. 1983;225:279-284148. Hertzberg RP, Dervan PB. Cleavage <strong>of</strong> DNA with methidiumpropyl-EDTA-iron(II):Reaction conditions and product analyses. Biochemistry. 1984;23:3934-3945149. Rollet-Labelle E, Grange MJ, Elbim C, Marquetty C, Gougerot-Pocidalo MA, Pasquier C.Hydroxyl radical as a potential <strong>in</strong>tracellular mediator <strong>of</strong> polymorphonuclear neutrophilapoptosis. Free Radic Biol Med. 1998;24:563-572150. Ikeda K, Kajiwara K, Tanabe E, Tokumaru S, Kishida E, Masuzawa Y, Kojo S.Involvement <strong>of</strong> hydrogen peroxide and hydroxyl radical <strong>in</strong> chemically <strong>in</strong>duced apoptosis <strong>of</strong>HL-60 cells. Biochem Pharmacol. 1999;57:1361-1365151. Gutteridge JM, Wilk<strong>in</strong>s S. Copper salt-dependent hydroxyl radical formation. Damage toprote<strong>in</strong>s act<strong>in</strong>g as anti<strong>oxidant</strong>s. Biochim Biophys Acta. 1983;759:38-41152. Halliwell B, Clement MV, Long LH. Hydrogen peroxide <strong>in</strong> the human body. FEBS Lett.2000;486:10-13153. Halliwell B, Gutteridge JM. Role <strong>of</strong> free radicals and catalytic metal ions <strong>in</strong> human disease:An overview. Methods Enzymol. 1990;186:1-85154. World Health Organization (WHO), Geneva, 2004. Guidel<strong>in</strong>es for dr<strong>in</strong>k<strong>in</strong>g-<strong>water</strong> quality,third edition. 2004155. The Council <strong>of</strong> the European Union. Council directive 98/83/ec on the quality <strong>of</strong> <strong>water</strong><strong>in</strong>tended for human consumption. Official Journal <strong>of</strong> the European Communities. 1998156. Boulay N, Edwards M. Role <strong>of</strong> temperature, chlor<strong>in</strong>e, and organic matter <strong>in</strong> coppercorrosion by-product release <strong>in</strong> s<strong>of</strong>t <strong>water</strong>. Water Res. 2001;35:683-69058
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Pro-oxidant activity of vitamin C i
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Supervised byDocent Tommy Nordströ
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ContentsCONTENTSLIST OF ORIGINAL PU
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List of original publicationsLIST O
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AcknowledgementsACKNOWLEDGEMENTSThi
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AbbreviationsABBREVIATIONSAsc …
- Page 14 and 15: Review of the literatureREVIEW OF T
- Page 16 and 17: Review of the literatureSince vitam
- Page 18 and 19: Review of the literaturestill added
- Page 20 and 21: Review of the literatureantioxidant
- Page 22 and 23: Review of the literatureThe α-toco
- Page 24 and 25: Review of the literatureCopper, wil
- Page 26 and 27: Review of the literatureOH • + H
- Page 28 and 29: Review of the literatureFormation o
- Page 30 and 31: Review of the literature3.2. The ro
- Page 32 and 33: Review of the literaturecopper conc
- Page 34 and 35: Experimental proceduresEXPERIMENTAL
- Page 36 and 37: Experimental procedures2.2. Measure
- Page 38 and 39: Experimental procedurestetrahydrate
- Page 40 and 41: ResultsRESULTS1. Vitamin C induces
- Page 42 and 43: Results3. Oxidative decomposition o
- Page 44 and 45: Resultsdifferent water samples vari
- Page 46 and 47: DiscussionDISCUSSIONNowadays, ascor
- Page 48 and 49: DiscussionCu 2+ + Asc → Cu + + As
- Page 50 and 51: Discussion3. Iron inhibits vitamin
- Page 52 and 53: Discussionperoxide might have an im
- Page 54 and 55: ConclusionsCONCLUSIONSThe main focu
- Page 56 and 57: ReferencesREFERENCES1. Arrigoni O,
- Page 58 and 59: References34. Padayatty SJ, Katz A,
- Page 60 and 61: References66. Sies H, Stahl W, Sund
- Page 62 and 63: References95. Halliwell B. Role of
- Page 66 and 67: References157. Critchley MM, Cromar
- Page 68 and 69: References185. Liao CH, Kang SF, Wu
- Page 70 and 71: References214. Orr CW. Studies on a
- Page 72: References243. Miller C, Kennington
- Page 88 and 89: Free Radical Research, Volume 38 Nu
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