The Clean Microscope

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Suppliers and RecipesKerma ophthalmic cotton N 1, DAB 6The cotton wool used in ophthalmology is 100% purecotton (DIN 61 640-A, Ph.Eur., DAB). It is absolutely pure,highly absorbent and soft. The fibers can be blown awayfrom optical surfaces.www.kerma.deWHATMAN Lens Cleaning Tissue 10510 cm x 15 cm lens tissue in packs containing 25 walletsof 25 sheets each, Catalog No. 2105 841.The only lens tissue recommended by Carl Zeiss. It ischemically pure and silicon-free, and contains absolutelyno additives. This product is also sold by other suppliers,e.g. KODAK.www.whatman.comRubber blowerThis is a particularly powerful blower:Giotto‘s, Type GTAA 1900.www.giottos.comCarl Zeiss optical cleaning solutionRecipe: 85% n-hexane, 15% isopropanol.The solution is not sold by Carl Zeiss MicroImagingGmbH.The n-hexane should be analytical grade. Some benzinefractions (petroleum ether) are not suitable, as they leavean insoluble film on the optical surface. Acetone, whichis recommendable exclusively for the occasional cleaningof oil-contaminated cover slips, should also be analyticalgrade.Cover glasses of specified thickness D = 0.17 mmTo avoid spherical aberration use cover glassesD = 0.17 mm. They are available from Carl Zeiss.Item discription: High-precision cover glasses,D = 0.17 mm, 18 x 18 mm, 1000 Pieces,Order-Nr. 474030-9000.ITW Texwipe CleanTipsAbsorbent polyester swabs forcleaning optical components(Alpha, Clean Foam or Absorbond series)Available in different sizes and absorption grades, e.g.,from Basan under designation TEXWIPE TX743B.www.texwipe.comwww.basan.comSAFETY WARNINGWhen working with chemicals, solvents andother possible hazards, be sure to follow thecurrent safety regulations applicable in yourcountry and/or institution.14
Subject IndexPageAcetone . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7, 8, 11, 17Acid vapors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13, 17Anisol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6Antireflection coatings . . . . . . . . . . . . . . . . . . . . . . . . 7, 11Benzine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8, 14Blue WINDEX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Camera adapters . . . . . . . . . . . . . . . . . . . . . . . 4, 7, 11, 17Cameras . . . . . . . . . . . . . . . . . . . . . . . . . . . 4, 5, 7, 10, 17Chloroform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7, 11Cleaner‘s naphtha . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Cleaning movement . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Cleaning procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10Cotton. . . . . . . . . . . . . . . . . . . . . . . 7, 8, 9, 10, 11, 14, 17Cover slips . . . . . . . . . . . . . . . . . . . . . 4, 5, 6, 8, 11, 12, 14Diethyl ether . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11, 17Dirt – loose, water-soluble, oily, greasy . . . . . . . . . . . . . . 10Dirt on cameras . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4, 5, 7Dirt on front lens . . . . . . . . . . . . . . . . . . . . . . . . . . . 4, 5, 7Dry objectives. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4, 6, 7Dry objective with correction collar . . . . . . . . . . . . . . . . . . 6Dust. . . . . . . . . . . . . . . . . . . . . . . . . . 5, 6, 7, 8, 10, 12, 13Ethanol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11, 17Fluorescence filter sets . . . . . . . . . . . . . . . . . . . . . . . . . 4, 7Fungus. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13IMMERSOL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4, 6Isopropanol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10, 14JENA Microscopes 250 CF . . . . . . . . . . . . . . . . . . . . . . . 17Kleenex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Locating dirt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5MIKROVAL microscopes . . . . . . . . . . . . . . . . . . . . . . . . . 17n-hexane . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8, 10, 11, 14Optical cleaning solution (Carl Zeiss) . . . . . . . . 8, 10, 11, 14Optical surfaces . . . . . . . . . . . . . . . . . . . . . . . . . 4, 7, 8, 17Painted surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7, 12Petroleum ether . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8, 14Polyester swabs . . . . . . . . . . . . . . . . . . . . . . . . . 7, 8, 9, 10Polystyrene sticks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17Rubber blower . . . . . . . . . . . . . . . . . . . . . . . . 8, 10, 14, 17SIDOLIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Solvents . . . . . . . . . . . . . . . . . . . . . . 6, 7, 8, 10, 11, 14, 17SPARKLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Spherical aberration . . . . . . . . . . . . . . . . . . . . . . . 6, 12, 14STANDARD microscopes . . . . . . . . . . . . . . . . . . . . . . . . . 17Swabs . . . . . . . . . . . . . . . . . . . . . . . 7, 8, 9, 10, 11, 14, 17WHATMAN tissue. . . . . . . . . . . . . . . . . . . . . . . . . . 8, 9, 14White spirit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Xylene . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1115
Page 3 and 4: The Clean MicroscopeDr. Michael Zö
Page 5 and 6: Recognizing and CleaningSoiled Opti
Page 7 and 8: How to Recognize DirtTo recognize d
Page 9 and 10: Different Optical SurfacesConcave o
Page 11: Preparation of cotton swabs■ Wash
Page 14 and 15: Cleaning External(Non-Optical) Micr
Page 18 and 19: Please contact the local Carl Zeiss
Page 20: Clean optics are essentialfor succe

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