Three new species of Oidiodendron Robak from Spain - CBS

More documents

Recommendations

Info

CALDUCH ET AL.irregularly folded, with a darkening brown(M6D86F8) reverse. Colonies on PCA were 2630mm diam, olive-brown (M4E44F5), flat, velvety,with an olive-brown (M4E44F5) reverse. Colonieson PDA were 3740 mm diam, greyish orange togreyish brown (M5B35F3), slightly fasciculate at thecentre, radially folded, with a brownish orange toyellowish brown (M5C55F5) reverse. The fungusshowed good sporulation on the three culture mediamentioned. Microscopic features under these conditionsdid not vary from those on the natural substrate.At 15 ºC the fungus grew more slowly than at 25 ºCon all media assayed, with colonies attaining 1826mm diam after 14 wk. A diffusible brownish orange tobrown (M6C76D8) pigment was produced only onOA. No growth was observed at 37 ºC.Specimen examined: Spain, Asturias, Muniellos IntegralNature Reserve, on adecaying basidiome, 26 June 1999,col. A.M. Stchigel & M. Calduch, holotype IMI 391998,ex-type culture CBS 113932 = FMR 6765.Figs 710. Oidiodendron muniellense (IMI 391998). 7.Habit sketch. 8. Conidiophore. 9. Fertile hyphae bearingarthroconidia. 10. Arthroconidia. Scale bars: 7 = 50 m, 8,9 = 10 m, 10 = 2.5 m.Hyphomycetes. Colonies on the natural substrateeffuse, hairy, greenish brown. Mycelium partly immersedin the substrate. Hyphae pale brown to brown,septate, 12 m wide. Conidiophores macronematous,mononematous, each composed of a long stipe and arepeatedly branched upper part from where fertilehyphae are borne; stipe straight, septate, brown,smooth- and thick-walled, up to 200 m long, 23.5m wide at the base; upper part with 46 verticillatebranches, each branch several times subdichotomouslyor trichotomously branched, seta-like, straight, septate,brown, paling distally, smooth- and thick-walled,becoming roughened and thin-walled towards theapex, pointed at the tip, up to 60 m long, 1.52.5 mwide near the base. Fertile hyphae terminal or lateralon the dark hyphae of the conidiophore upper part,branched, septate, subhyaline, smooth-walled, cylindrical,12.5 m wide, fragmenting to form arthroconidia.Conidia dry, catenate, one-celled, globose tosubglobose, ochraceous, roughened (echinulate underSEM), thin-walled, 1.52.5 m diam. Teleomorphunknown.Cultural characteristics: The optimal colony growthwas at 25 ºC. At this temperature and after 4 wk ofincubation, colonies on OA were 3035 mm diam,brownish beige to brown (M6E36E8), flat, velvety,Notes: The present species is similar to O. hughesiiUdagawa & Uchiyama (Udagawa & Uchiyama 1998)and O. setiferum (Udagawa & Toyazaki 1987) in themorphology of the terminal part of the conidiophores.However, O. hughesii differs mainly by the developmentof a reticulum of dark olive-brown and conspicuouslyornamentated hyphae with spine-likeappendages at the periphery and by the production ofonly lateral fertile hyphae from the hyphal reticulum.The upper part of the conidiophore of O. setiferum ischaracterized by a subterminal whorl of dark brown,sterile setiform branches, which surrounds a terminalwhorl of subhyaline penicillate fertile hyphae. Moreover,conidia of O. muniellense are globose to subglobose,roughened and ochraceous, while those of O.setiferum and O. hughesii are ovoid to ellipsoidal, andsmooth-walled and brown, paler in the former, andasperate and hyaline in the latter.Oidiodendron ramosum Calduch, Stchigel, Gené& Guarro, sp. nov. MycoBank MB500041. Figs1122.Etymology: Referring to the branched conidiophores.Ad Hyphomycetes pertinens. Coloniae in basidiomateputrido, pilosae, olivaceo-brunneae coloratae. Hyphispallide brunneis vel brunneis, septatis, 1.52 m latis.Conidiophora macronematosa, mononematosa, cum stipitesimplici, recto, septato, brunneo, plerumque verrucoso,crassitunicato, usque ad 150 m longo, 23 m lato adbasim, rami sursum in hyphas fertiles terminantes; raminumero 26, subdichotomi, septati, longi recurvati, apicemversus attenuati, verruculosi vel verrucosi, pallidioresbrunnei ad basim, palliodore ad apicem, usque ad 130 m162
NEW SPECIES OF OIDIODENDRONlongi, 1.52.5 m lati ad basim. Hyphae fertiles terminalesvel laterales, ramosae, septatae, laeves, cylindricae, subhyalinae,11.5 m latae. Conidia sicca, catenata, unicellularia,subglobosa vel ellipsoidea, pallide brunnea, laevia velleniter rugosa, tenuitunicata, 1.53 12 m. Teleomorphosisignota.Hyphomycetes. Colonies on a decaying basidiome,hairy, greenish brown. Mycelium partly immersed inthe substrate. Hyphae pale brown to brown, septate,1.52 m wide. Conidiophores macronematous,mononematous, composed of a stipe bearing at theupper part a whorl of dematiaceous, fertile seta-likebranches which surrounds a dense head of fertilehyphae; stipe straight, septate, brown, verruculose,thick-walled, up to 150 m long, 23 m wide at thebase; upper part with 26 verticillate branches, eachbranch subdichotomously branched, seta-like, septate,broadly recurved, attenuate at the tip, verruculose toverrucose, pale brown and becoming subhyalinedistally, up to 130 m long, 1.52.5 m wide near thebase, from which fertile hyphae also develop. Fertilehyphae terminal or lateral on the dematiaceousbranches of the conidiophore upper part, but also onthe terminal portion of the conidiophore stipe, forminga dense whorl of penicillate hyphae, septate, smoothwalled,subhyaline, cylindrical, 11.5 m wide,fragmenting to form arthroconidia.Figs 1118. Oidiodendron ramosum (IMI 391997). 11, 12. Conidiophores on the natural substrate. 13. Detail of a conidiophoreapical part with fertile ramifications that surround arthroconidial masses. 14. Conidiophore in vitro. 15, 16. Penicillate fertilehyphae. 17, 18. Arthroconidia. Scale bars: 11, 12 = 100 m, 13 = 25 m, 14 = 50 m, 15, 17 =10 m, 16 = 5 m, 18 = 2.5 m.163
Page 2 and 3: CALDUCH ET AL.Nature Reserve. The M
Page 6 and 7: CALDUCH ET AL.Conidia dry, catenate
Page 8 and 9: CALDUCH ET AL.Figs 3235. Oidiodendr
Page 10 and 11: CALDUCH ET AL.5. Branches anastomos
Page 12: CALDUCH ET AL.Udagawa S, Toyazaki N

Three new species of Oidiodendron Robak from Spain - CBS

Create successful ePaper yourself

Delete template?

Save as template?