Three new species of Oidiodendron Robak from Spain - CBS

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CALDUCH ET AL.Conidia dry, catenate, one-celled, subglobose toellipsoidal, pale brown, smooth to slightly roughened(roughened under SEM), thin-walled, 1.53 12m. Teleomorph unknown.Cultural characteristics: The optimal colony growthwas at 25 ºC. Colonies on OA were 2025 mm diamafter 4 wk, olive-brown (M4F8), with greyish margins,flat, powdery, concentrically radiate, with anolive-brown (M4D54E5) reverse. On PCA colonieswere 1820 mm diam after 4 wk, olive (M2F4) withwhitish margins, flat, velvety, with an olive (M2E4)reverse. On PDA, they were 2730 mm diam after 4wk, orange-white to greyish orange (M5A25B3),cerebriform, radially folded, with a greyish orange(M5B45B5) reverse. The microscopic features of thefungus did not vary from those observed on the naturalsubstrate. At 15 ºC the fungus grew slower (1317mm diam after 4 wk) and sporulation was poorer thanat 25 ºC on all the media assayed. No growth wasobserved at 37 ºC.Figs 1922. Oidiodendron ramosum (IMI 391997). 19.Habit sketch. 20. Conidiophore. 21, 22. Fertile hyphaebearing arthroconidia. Scale bars: 19 = 100 m, 20, 21 = 20m, 22 = 4 m.Specimen examined: Spain, Asturias, Muniellos IntegralNature Reserve, on an unidentified rotten basidiocarp, 26June 1999, coll. A.M. Stchigel & M. Calduch, holotypeIMI 391997, ex-type cultures CBS 113931 = FMR 6764.Notes: Oidiodendron setiferum (Udagawa & Toyazaki1987) is most similar to O. ramosum. Both have at the164upper part of the conidiophore stipe a dense head offertile hyphae surrounded by a whorl of dematiaceous,seta-like branches. However, in the former, thesebranches are sterile, while in O. ramosum they arefertile. Moreover, the conidia of O. setiferum aresmooth-walled and separated each other by thick anddark connectives. Oidiodendron muniellense alsoresembles O. ramosum in conidiophore morphology,but in the former the peripheral branches of the conidiophoreupper part are strainght and smoothwalled,becoming roughened distally. Additionally,the conidial morphology of O. muniellense is different.Oidiodendron reticulatum Calduch, Stchigel,Gené & Guarro, sp. nov. MycoBank MB500042.Figs 2335.Etymology: Referring to the morphology of the upperpart of the conidiophores.Ad Hyphomycetes pertinens. Coloniae in vitro 2225 ºCrestrictae, planae, olivaceo-griseae coloratae. Hyphis pallidebrunneis, septatis, 11.5 m latis. Conidiophora macronematosa,mononematosa, stipite simplici, reticulum globosumin parte apicale formantes et hyphis lateralibus fertilibus;stipites reticulum e ramis anastomosantibus, 90150m diam, ramis apicem versus attenuatis et rotundatis,septatis, fortiter verrucosis et spinulosis, brunneis, 560 mlongis, 23 m latis ad basim. Hyphae fertiles ex hyphisreticuloriundae, ramosae, septatae, laeves, subhyalinae velpallide griseo-brunneae, cylindricae, 515 m longis, 1.52m latae. Conidia sicca, catenata, unicellularia, ellipsoidea,pallide brunnea vel pallide olivaceo-brunnea, rugosa velechinulata, tenuitunicata, 1.54 1.52.5 m. Conidiophoraaltera simplicia; co-formantia, stipites recti, septati,leniter crassitunicati, pallide brunnei, usque ad 350 mlongi, 1.52.5 m lati ad basim; capitulum compactumhypharum fertilium ferentes. Teleomorphosis ignota.Hyphomycetes. Colonies in vitro at 2225 ºC restricted,plane, olive-grey in colour. Mycelium partlyimmersed in the substrate, composed of pale brown,septate, 11.5 m wide hyphae. Conidiophoresmacronematous, mononematous, of two types: i) astipe ending in a reticulate network of hyphae fromwhich lateral fertile hyphae are produced; stipestraight, septate, brown, smooth-walled to slightlyverruculose, thick-walled, up to 200 m long, 1.52.5m wide at the base, upper part several timesbranched and anastomosing to form a more or lessglobose reticulate network, measuring 90150 mdiam, with seta-like peripheral branches; branches ofthe reticulum recurved or sinuous, attenuate androunded at the tip, septate, strongly verrucose andspinulose throughout, brown, 560 m long, 23 mwide at the base; and ii) conidiophores simple, stipestraight, septate, slightly thick-walled, pale to brown,
NEW SPECIES OF OIDIODENDRONup to 350 m long, 1.52.5 m wide at the base,producing terminally a dense head of fertile hyphae.Fertile hyphae arising laterally from branches of thereticulum or terminally from the conidiophore stipe,branched, septate, smooth-walled, subhyaline to palegreyish brown, cylindrical, 515 m long, 1.52 mwide, fragmenting to form arthroconidia. Conidia dry,catenate, one-celled, pale brown to pale olivaceousbrown,roughened (echinulate under SEM), thinwalled,ellipsoidal, 1.54 1.52.5 m, with one orboth ends truncate. Teleomorph unknown.Cultural characteristics: The optimal colony growthwas at 25 ºC. Colonies on OA were 2932 mm diamafter 4 wk, with abundant conidiophores arranged inconcentric rings, brownish grey (ME34F3), flat,slightly granulose; reverse brownish grey(M4E34F3); a diffusible pigment brownish orange tobrown (M6C66E8). On PCA, colonies 2025 mmdiam after 4 wk, olive-grey (M3E23F2), with whitishmargins, flat, velvety; reverse olive-grey. On PDA,3035 mm diam after 4 wk, pale yellow to greyishyellow (M1A31B4), olive (M1E41F4) at the centre,slightly fasciculate, irregularly folded; reverse orangegreyto greyish orange (M5B25B3). At 15 ºC colonygrowth rates on OA and PDA were similar to those at25 ºC, while on PCA they were restrictedly (1216mm in 4 wk), sporulation was scarce and the diffusiblepigment was absent in all media assayed. No growthwas observed at 37 ºC.Figs 2331. Oidiodendron reticulatum (IMI 391999). 2325. Two different type of conidiophores, simple on the left and withan apical reticulate network of hyphae on the right. 2628. Network wall ornamentation. 29, 30. Fertile hyphae producingarthroconidia. 31. Arthroconidia. Scale bars: 23, 25 = 50 m, 24 = 30 m, 2628, 30 =10 m, 29 = 20 m, 31 = 2 m.165
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Three new species of Oidiodendron Robak from Spain - CBS

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