A new species and re-disposed taxa in Repetophragma - CBS

MycosphereA new species and re-disposed taxa in RepetophragmaCastañeda-Ruiz RF 1 , Heredia G 2 , Arias RM 2 , McKenzie EHC 3 , Hyde KD 4 , Stadler M 5* ,Saikawa M 6 , Gené J 7 , Guarro J 7 , Iturriaga T 8 , Minter DW 9 and Crous PW 101 Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro de Humboldt”, Calle 1 Esq. 2, Santiagode Las Vegas, C. Habana, Cuba, C.P. 172002 Instituto de Ecología, A.C., Congregación El Haya, Xalapa, Veracruz 91070, Mexico3 Landcare Research, Private Bag 92170, Auckland, New Zealand4 School of Science, Mac Fah Luang University, Thasud, Chiang Rai 57100, Thailand5 University of Bayreuth, Dept. Mycology, Universitätstr. 30, D-95544 Bayreuth, Germany6 Tokyo Gakugei University, Dept. Biology, Nukuikita-machi, Koganei-shi, Tokyo 184-8501, Japan7 Unitat de Microbiologia, Facultat de Medicina i Ciencies de la Salut, Universitat Rovira i Virgili, 43201 Reus,Tarragona, Spain8 Departamento Biología de Organismos, Universidad Simón Bolívar, Apartado 89000. Sartenejas, Baruta, Edo Miranda,Venezuela9 CABI, Bakeham Lane, Egham, Surrey, TW20 9TY, United Kingdom10 CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The NetherlandsCastañeda-Ruiz RF, Heredia G, Arias RM, McKenzie EHC, Hyde KD, Stadler M, Saikawa M,Gené J, Guarro J, Iturriaga T, Minter DW, Crous PW. 2011 – A new species and re-disposed taxa inRepetophragma. Mycosphere 2(3), 273–289.A new species of Repetophragma, collected on plant material from “Aguita Fria” Veracruz, Mexico,and from Canaima National Park, Bolivar State, Venezuela is described and illustrated.Repetophragma paracambrense sp. nov. is characterized by subobclavate to somewhat lageniform,truncate at the base, 8−10-septate, brown, mostly verruculose, sometimes smooth conidia. Twelvenew combinations are proposed for taxa previously included in Sporidesmium and Endophragmiella.A comparative table and key to Repetophragma species are provided.Key words – anamorphic fungi – cloud forest – Guiana forest – systematicsArticle InformationReceived 17 Febuary 2011Accepted 10 May 2011Published online 9 August 2011*Corresponding author: Marc Stadler – e-mail – marc.stadler@t-online.deIntroductionSubramanian (1992) erected the genusRepetophragma during a revision of SporidesmiumLink, and included nine species. Repetophragmabiseptata (M.B. Ellis) Subram. wasdesignated as the type species. The diagnosticcharacters of the genus are macronematousconidiophores with several annellations producedafter a few or numerous enteroblasticpercurrent proliferations of the conidiogenouscell, and euseptate conidia with a conicotruncatebasal cell, which seccede schizolytically.The combination of these featuresseparates the genus Repetophragma fromSporidesmiella P.M. Kirk, which has distoseptateconidia (McKenzie 1995). Subsequently,ten other species have been added to thegenus: Repetophragma cambrense (M.B. Ellis)McKenzie (McKenzie 1995), R. cubense(Mercado) J. Mena (Mena et al. 2000), R. goidanichii(Rambelli) W.P. Wu (Wu & Zhuang2005), R. inflatum (Berk. & Ravenel) W.P. Wu(Wu & Zhuang 2005), R. ontariense (Matsush.)W.P. Wu (Wu & Zhuang 2005), R. sinenseW.P. Wu (Wu & Zhuang 2005), R. fasciatum(R.F. Castañeda) R.F. Castañeda, Gusmão &273

Saikawa (Castañeda et al. 2006), R. filiferum(R.F. Castañeda) R.F. Castañeda et al.(Castañeda et al. 2006), R. calongei J. Mena,Silvera & Gené (Silvera-Simón et al. 2009, andR. gondwanamycetis Marinc., M.J. Wingf. &Crous (Marincowitz et al. 2008).During an expedition in February 2010through the protected areas of Veracruz State,Mexico, a conspicuous fungus from the genusRepetophragma was collected on decayingwood. A specimen collected in a tropical forestin southern Venezuela during 2005 proved tobe the same fungus. These specimens showeddifferences from previously described taxa andare herein described and illustrated as a newspecies of Repetophragma.Materials and methodsDuring February 2010 around 80 samplesof microfungi were collected in “Aguita Fria”,Veracruz, Mexico (Fig. 21). Individual collectionswere placed in paper bags, taken to thelaboratory, and incubated at 25°C in Petridishes placed in a moist chamber composed ofplastic containers (50 L capacity) with 200 mLof sterile water plus 2 mL of glycerol, andexamined at regular intervals for the presenceof microfungi. Aeration was supplied by openingthe containers for 5 to 10 minutes at 45minutes intervals during a week. Mounts wereprepared in polyvinyl alcohol-glycerol (8.0 g in100 mL of water, plus 5 mL of glycerol) andmeasurements made at a magnification of×1000. The same methodology was followedfor specimens collected during an expedition inApril 2005 to Luepa, Gran Sabana area,Canaima National Park, as part of a series ofexpeditions to collect wood decomposing fungiin a tropical forest in southern Venezuela.Micrographs were obtained with a ZeissAxioskop 40 microscope and a Leitz Dialux 20EB microscope. Holotype material is depositedin the Herbarium of the “Instituto de EcologíaA.C., Xalapa (XAL) and a paratype at HerbarioNacional de Venezuela in Caracas (VEN).TaxonomyRepetophragma paracambrense R.F. Castañeda, Heredia & McKenzie, sp. nov.Figs 1−20MycoBank 519657Etymology − Greek, para- similar to, bythe side of; cambrense, epithet of similarspecies in the genus Repetophragma.Figs 1−19 − Repetophragma paracambrense,photomicrographs from holotype (XALCB1700). 1−12 Conidia. 13−19 Conidiogenouscells and conidia. Scale bars = 10 µm.Coloniae in substrato naturali effusae,pilosae, nigrae vel atrobrunneae. Myceliumpartim superficiale et partim in substrato immersum,ex hyphis septatis, ramosis, brunneis,verruculosis vel laevibus. Conidiophora macronematosa,mononematosa, erecta, recta velleviter geniculata, 5−8-septata, sursum perproliferata,ad usque 10 proliferationes successivas,180−350 × 7−10 µm, atrobrunnea adbasim, pallidiora ad apicem. Cellulae conidiogenaemonoblasticae, terminales, in conidiophorisincorporatae, indeterminatae, cum proliferationesenteroblasticis percurrentis deinde274

MycosphereFig. 20 − Repetophragma paracambrense, drawings from holotype (XAL CB1700). Conidiophores,conidiogenous cells and conidia. Scale bar = 10 µm.aliquot cyathiformibus et annuliformibus visae.Conidiorum secessio schizolytica. Conidiasolitaria, subobclavata vel aliquot lageniformia,truncata ad basim, leviter constricta ad cellulasubapicale et rotundata ad apicem, 8−10-septata,utrimque laevia et subhyalina, verruculosaet brunnea cetero, sed atrobunnea vel nigra adsepta, sicca, 35−55 × 10−13 µm. Teleomorphosisignota.Colonies on the natural substratum effuse,hairy, black or dark brown. Myceliumsuperficial and immersed, composed of septate,branched, brown, verruculose or smooth hyphae.Conidiophores macronematous, mononematous,simple, erect, straight or slightly geniculatetowards the apex, 5−8-septate, annellateabove, with up to 10 annellations, 180−350 ×7−10 µm, dark brown at the base and palebrown towards the apex. Conidiogenous cellsmonoblastic, terminal, integrated, indeterminate,annellidic with numerous cyathiformenteroblastic percurrent proliferations. Conidialsecession schizolytic. Conidia solitary, subobclavateto somewhat lageniform, truncate at thebase, slightly constricted at the subapical celland rounded at the apex, 8−10-septate, septablack, subhyaline and smooth at the ends, othercells verruculose and brown, 35−55 × 10−13µm, dry. Teleomorph unknown.Habitat − on decaying twig of unidentifiedplant, on fallen decomposing decorticatedbranch.Known distribution − Mexico, Venezuela.Material examined: Mexico, Veracruz, “Aguita275

MycosphereRepetophragma quadriloculare (Matsush.)R.F. Castañeda, McKenzie & K.D. Hyde,comb. nov. Fig. 52MycoBank 519668Basionym − Endophragmiella quadriloculareMatsush, Matsushima MycologicalMemoirs 7: 51, 1993.Repetophragma terminaliae (Deighton) R.F.Castañeda, McKenzie & K.D. Hyde, comb.nov. Fig. 56MycoBank 519669Basionym − Sporidesmium terminaliaeDeighton, in Deighton & Cejp, MycologicalPapers 117: 25, 1969.The ability to generate one or numerouselongations of the conidiogenous cells can beused to separate taxa at the generic level, butcannot be used to differentiate species, sincethe number of proliferations (annellations) canbe influenced by environmental conditions.Wang (1990) pointed out that “the annellationson an annellophore are the results of theregeneration of entire new conidiogenous cells,originating either from the penultimate cells orfrom endohyphae”, it can be confirmed withthe pale or colorless pigmentation of apicalproliferation that characterized new materialformed during the conidiogenous events andregeneration of the conidiogenous cells.To assist in identification of species asynoptic table with the principal diagnosticfeatures is provided (Table 1), along with a keyto the species.Table 1 Comparison of described and re-disposed Repetophragma species.Species Conidiophores Conidia ReferencesMorphology and size (µm) Morphology and size (µm)R. aburiense Brown, up to 14annellations, 7−80 × 4−6R. afrormosiae Pale brown or olivaceousbrown, up to 5 annellations,8−30 × 4−6R. apiceinflatum Pale brown, subhyalinetowards apex, 1−7annellations, 20−45(−110) ×3 −4R. biseptatum Mid to dark brown, 0−18-annellations, 55−190 ×4.5−6R. calongei Brown to dark brown, 4−9annellations, 32−75 × 5−7R. cambrense Reddish brown or darkbrown, paler towards apex,up to 6 annellations, 60−300× 7−11R. cubense Dark brown, 3−17annellations, 50−180 × 6−9Obclavate, rostrate, 2−4-septate, brown, palertowards apex, smooth orverruculose, 40−65 × 6−7Obclavate, often rostrate andcurved, 7−12-septate,sometimes 1−2 obliquesepta, brown, paler towardsapex, smooth, 60−100 ×8−10Cylindrical, globose toslightly inflated at apex,4−12-septate, pale brownwith hyaline basal cell,smooth, 27−80(−110) ×4−5.5Navicular, 2-septate, brownto dark brown, basal cellsubhyaline, smooth, 17−23 ×7−8Subcylindrical tosubfusiform, 12−27-septate,brown to mid brown,smooth, 80−165 × 8−10.5Subfusiform, 7−10-septate,brown with paler end cells,smooth, (47−)53−63(−70) ×10−14Obclavate, rarely fusiform,rostrate, 6−13-septate, darkgolden brown, pale at baseand towards apex, obscureband at septa, smooth,50−200 × 13−18, rostrum upto 130 longEllis (1958, 1976),Subramanian (1992)Ellis (1963, 1976),Subramanian (1992)Matsushima (1995)Ellis (1963, 1976),Subramanian (1992)Silvera-Simón et al.(2009)Ellis (1958),McKenzie (1995)Mercado (1984a, b),Mena-Portales et al.(2000)277

Table 1 (Continued) Comparison of described and re-disposed Repetophragma species.Species Conidiophores Conidia ReferencesMorphology and size (µm) Morphology and size (µm)R. dennisii Brown, pale brown towardsapex, 7−27 annellations,80−310 × 6−8Ellipsoid to oval, (2−)3−4-septate, brown, apical cellpale brown, smooth, 23−32Kirk (1983), Subramanian(1992), Castañeda-Ruiz etal. (2003), Wu & ZhuangR. ellisii Yellow-brown near base,pale straw towards apex,3−8 annellations, 60−180 ×3.5−5.0R. fasciatum Dark brown, pale browntowards apex, 4−8annellations, 100−150 × 5−9R. filiattenuatum (*) Dirty brown, pale at apex,up to 7 annellations, 30−150× 6−10R. filiferum Brown, 4−10 annellations,60−100 × 4−6R. filisporum (**) Dirty brown, pale towardsapex, up to 4 annellations,20−50(−100) × 3.5−5R. fragmentisporum (**) Dirty brown, pale near apex,3−6 annellations andsometimes with somesympodial elongations, up to40 × 3−4R. goidanichii Brown to dark brown, 5−11annellations, 40−78 × 5−8R. gondwanamycetis Brown, pale near apex, 0−1annellations, 70−160 × 5−6R. indicum Dark brown, 7−12annellations, 50−100 × 5−8× 11−13Obclavate, acicular rostrate,4−5-septate, yellow-brown,two middle cells darkerstraw-coloured, apical cellhyaline, basal cellsubhyaline, smooth, 40−90 ×7−9, rostrum up to 60 longEllipsoid to oval, with afiliform, straight apicalappendage, 5-septate,brown, dark brown at middlecells, black band at septa,smooth, 22−27 × 10−12,appendage 80−90 longObclavate, cylindricalrostrateabove, 7−23-septate,brown, subhyaline towardsapex, smooth, 80−520 ×10−16, rostrum up to 400long. On natural substratum68−165 × 12−14Broad fusiform to navicular,with a filiform, straight orflexuous apical appendage,6−8-septate, mid palebrown, smooth, 28−40 ×8−10, appendage up to 100longNarrow obclavate tosubcylindrical, 6−40-septate,dirty brown, pale brown orsubhyaline towards apex,smooth, 50−300 × 4−5. Onnatural substrate 27−70 ×4−5.5Cylindrical, 6−20-septate,pale olivaceous, smooth,36−85 × 4−5Cylindrical, 7−9-septate,brown, smooth, 40−52 ×5−7Cylindrical or fusiform,slightly curved or straight,3−12-septate, pale to darkbrown, subhyaline end cells,smooth, (25−)31–34(−45) ×5−9Obclavate, slightlyconstricted at septum, 1-septate, pale golden brown,smooth, 30−42 × 7−9(2005)Pirozynski (1972), Ellis(1976)Castañeda-Ruiz (1985),Grandi & Silva (2003),Castañeda-Ruiz et al.(2006)Matsushima (1983),Subramanian (1992),Wu & Zhuang (2005)Pirozynski (1972), Ellis(1976), Castañeda-Ruiz etal. (2006)Matsushima (1993)Matsushima (1993)Hughes (1979), Wu &Zhuang (2005)Marincowitz et al. (2008)Ellis (1976), Subramanian(1992)278

Table 1 (Continued) Comparison of described and re-disposed Repetophragma species.MycosphereSpeciesConidiophoresMorphology and size (µm)R. inflatum Brown, 4−8 annellations,80−400 × 6−9R. laxisporum Brown, up to 10annellations, 25−85 ×3.5−7.0R. longiphorum Dark brown, brown towardsapex, up to 14 annellations,350−800 × 7−10R. moniliforme Brown, dark brown nearbase, 1−4 annellations, 9−50× 4−5R. omahutaense (***) Brown, with 3−10-annellations, up to 70 longand 3−4 wideR. ontariense Pale brown to brown, with6−14-annellations, 30−50 ×5−7R. paracambrense Dark brown at the base andpale brown towards theapex, with up to 10-annellations, 180−350 ×7−10R. perproliferatum Brown, pale brown towardsthe apex, with 1−3-annellations, up to 70 longand 3−4 wideR. peruamazonicum (***) Brown, with 1−4-annellations, 25−65 × 4−6R. quadriloculare (***) Brown, pale brown towardsapex, 2−5-annellations, up to70 × 3−4ConidiaMorphology and size (µm)Obclavate, sigmoid, 3−5-septate, dark reddish-brownor dark brown second andoccasionally third cells, palebrown or subhyaline othercells, smooth, 45−90 ×11−18Fusiform to obclavate,subulate at apex, 6−7-septate, brown, end cellssubhyaline, smooth, 25−39 ×7−9Obclavate, rostrate, 3−6-septate, frequently 4-septate,median cells transverselystriate and brown, end cellssmooth and pale brown,45−85 × 9−10Moniliform, 8−22-septate,brown and verrucose at baseand middle body, palebrown and smooth towardsapex, 55−280 × 6−10Cylindrical, 2−5-septate,pale brown, smooth, 8−28 ×4−5Fusiform or obclavate,8−11-septate, pale brown tomid brown, smooth,100−140 ×15−20Sub-obclavate to somewhatlageniform, truncate at thebase, slightly constricted atthe subapical cell androunded at the apex, 8−10-septate, subhyaline andsmooth at the ends, othercells verruculose and brown,with black septa, 35−55 ×10−13Obclavate, with a narrow,tapered apical appendage,2−3-septate, second cellbrown, the other cells paler,smooth, 18−35 × 3, apicalcell 6−21 longObclavate to slightlycylindrical, 5−8-septate,brown and pale brown at theend cells, smooth, 23−32 ×6−8Obovate to subglobose, 3-septate, dark brown middleand basal cells, pale brownat apex, smooth, 16−21 ×8−13.On natural substrate 20−24× 11−14ReferencesEllis (1958, 1971),Matsushima (1975), Wu &Zhuang (2005)Castañeda-Ruiz (1985)Castañeda-Ruiz &Kendrick (1991)Matsushima (1993)Matsushima (1985)Wu & Zhuang (2005)Present paper.Castañeda-Ruiz &Kendrick (1991)Matsushima (1993)Matsushima (1993)279

Table 1 (Continued) Comparison of described and re-disposed Repetophragma species.SpeciesConidiophoresMorphology and size (µm)ConidiaMorphology and size (µm)R. sinense Pale brown to brown, 4−5- Obclavate, rostrate, slightlyannellations, 40−150 × 4−6 curved, 6−9-septate, palebrown to mid brown, smooth,35−45 × 7−9R. subulatum Olivaceous brown, up to 7 Obclavate to subfusiform,annellations, 20−90 × 8−10 7−10-septate, dark olivaceousbrown, pale brown towardsapex, smooth, 90−160 ×15−19R. terminaliae Pale olivaceous, up to 8 Cylindrical, curved, 4−5-annellations, 20−80 × 4−5.5 septate, pale olivaceous,smooth, 50−75 × 6.5−8R. wroblewskii Pale to mid golden brown, Obclavate or subfusiform,up to 8 annellations, 7−40 × 3−4-septate, pale to mid5−8golden brown, smooth orverruculose, 30−46 × 9−11R. zambiense Pale olivaceous, slightly Subcylindrical, often curved,sinuous, 2−5 annellations, slightly wider at middle cells,11−50 × 4−6.54−13-septate, occasionallywith a longitudinal septum inone or two central cells, paleolivaceous to deep olivaceous,smooth, 39−104 × 7−11(*) On V8 agar media (**) On banana leaf piece (***) On CMA mediaReferencesWu & Zhuang (2005)Ellis (1958, 1976),Subramanian (1992)Deighton (1969), Ellis(1976)Ellis (1958, 1976),Subramanian (1992)Deighton (1969), Ellis(1976), Subramanian(1992)Key to Repetophragma species1 Conidia 1-septate, obclavate, slightlyconstricted at the septum, pale goldenbrown, smooth, 30−42 × 7−92 µm ...........................................R. indicum (Fig. 42)Conidia with more than 1 septum..........22(1) Conidia with an apical rostrum (rostrate)...............................................................3Conidia without an apical rostrum (notrostrate or tapering to the apex).............83(1) Conidia usually with fewer than 6 septa.................................................................4Conidia usually with more than 5 septa.54(3) Conidia obclavate, with a narrow, taperedapical appendage, 2−3-septate, secondcell brown, other cells paler, smooth,18−35 × 3 µm, apical cell 6−21 µm long....................R. perproliferatum (Fig. 50)Conidia obclavate, 2−4-septate, brown,paler towards apex, smooth orverruculose, 40−65 × 6−7 µm ...............................................R. aburiense (Fig. 22)Conidia obclavate, acicular, 4−5-septate,yellow-brown, with two middle cellsdarker straw-coloured, apical cell hyaline,basal cell subhyaline, smooth, 40−90 ×7−9 µm, rostrum up to 60 µm long ......R.ellisii (Fig. 34)Conidia obclavate or subfusiform, 3−4-septate, pale to mid golden brown,smooth or verruculose, 30−46 × 9−11µm.......................... R. wroblewskii (Fig. 57)Conidia ellipsoid to oval, with a filiform,straight apical appendage, 5-septate,brown and dark brown at the middle cells,with a black band at the septa, smooth,22−27 × 10−12 µm, appendage 80−90µm long................. R. fasciatum (Fig. 35)5(3) Conidia usually with fewer than 10 septa............................................................... 6Conidia sometimes with more than 10septa....................................................... 76(5) Conidia obclavate, 3−6-septate, frequently4-septate, median cells transverselystriate and brown, end cells smooth andpale brown, 45−85 × 9−10 µm ..........................................R. longiphorum(Fig. 46)280

MycosphereConidia broad fusiform to navicular, witha filiform, straight or flexuous apicalappendage, 6−8-septate, mid pale brown,smooth, 28−40 × 8−10 µm, appendage upto 100 µm long........R. filiferum (Fig. 37)Conidia obclavate, slightly curved, 6−9-septate, pale brown to mid brown,smooth, 35−45 × 7−9 µm ..........................................................R. sinense (Fig. 53)Conidia fusiform to obclavate, subulateat the apex, 6−7-septate, brown, withsubhyaline end cells, smooth, 25−39 ×7−9 µm................R. laxisporum (Fig. 45)7(5) Conidia obclavate, curved, 7−12-septate,sometimes 1−2 oblique septa, brown,paler towards the apex, smooth, 60−100× 8−10 µm ........ R. afrormosiae (Fig. 23)Conidia obclavate, rarely fusiform, 6−13-septate, dark golden brown, pale at thebase and towards the apex, with obscureband at the septa, smooth, 50−200 ×13−18 µm, rostrum up to 130 µm long ....................................R. cubense (Fig. 29)Conidia obclavate, cylindrical-rostrateabove, 7−23-septate, brown and subhyalinetowards the apex, smooth, 80−520 ×10−16 µm, rostrum up to 400 µm long .......................... R. filiattenuatum (Fig. 36)8(2) Conidia mostly cylindrical or subcylindrical,not curved ...................................9Conidia mostly cylindrical or subcylindrical,curved .......................................10Conidia mostly obclavate, not curved ornot sigmoid, 6−40-septate, dirty-brown,pale brown or subhyaline towards theapex, smooth, 50−300 × 4−5 µm.......................................... R. filisporum (Fig. 38)9(8) Conidia 4−5-septate, pale olivaceous,smooth, 50−75 × 6.5−8.0 µm .......................................... R. omahutaense (Fig. 48)Conidia 7−9-septate, brown, smooth,40−52 × 5−7 µm ................................................................. R. goidanichii (Fig. 40)Conidia globose to slightly inflated at theapex, 4−12-septate, pale brown withhyaline basal cell, smooth, 27−80(−110)× 4.0−5.5 µm ..R. apiceinflatum (Fig. 24)Conidia 6−20-septate, pale olivaceous,smooth, 36−85 × 4−5 µm .......................................... R. fragmentisporum (Fig. 39)Conidia 12−27-septate, brown to midbrown, smooth, 80−165 × 8−10.5 µm.....................................R. calongei (Fig. 26)10(8) Conidia 4−5-septate, pale olivaceous,smooth, 50−75 × 6.5−8.0 µm ............................................. R. terminaliae (Fig. 56)Conidia slightly wider above the middlecells, 4−13-septate, occasionally with alongitudinal septum in one or two centralcells, pale olivaceous to deep olivaceous,smooth, 39−104 × 7−11µm ..................................................R. zambiense (Fig. 58)Conidia 3−12-septate, pale to dark brown,with subhyaline end cells, smooth,(25−)31−34(−45) × 5−9 µm .....................................R. gondwanamycetis (Fig. 41)11(8) Conidia 5−8-septate, brown and palebrown at the end cells, smooth, 23−32 ×6−8 µm ..... R. peruamazonicum (Fig. 51)Conidia 3−5-septate, dark reddish-brownor dark brown second and occasionallythird cells, other cells pale brown orsubhyaline, smooth, 45−90 × 11−18 µmR.inflatum...................................... (Fig. 43)Conidia 7−10-septate, dark olivaceousbrown and pale brown towards the apex,smooth, 90−160 × 15−19 µm .........................................R. subulatum (Fig. 54, 55)12(8) Conidia 8−11-septate, pale brown to midbrown, smooth, 100−140 × 15−20 µm.................................R. ontariense (Fig. 49)Conidia 7−10-septate, brown with palerend cells, smooth, (47−)53−63 (−70) ×10−14 µm .....R. cambrense (Figs 27−28)Conidia slightly constricted at the subapicalcell and rounded at the apex, 8−10-septate, subhyaline and smooth end cells,verruculose, and brown in the other cells,with black septa, 35−55 × 10−13 µm...................... R. paracambrense (Figs 1−20)13(8) Conidia navicular 2-septate, with subhyalinebasal cell, other cells brown to darkbrown, smooth, 17−23 × 7−8 µm............281

Fig. 21 − Dr. Vinicio Sosa during a foray in “Aguita Fria” cloud forest, Veracruz, Mexico, takingsome pictures from the samples.............................R. biseptatum (Fig. 25)Conidia not navicular ..........................1414(13)Conidia ellipsoid to oval, (2−)3−4-septate, brown, apical cell pale brown,smooth, 23−32 × 11−13 µm .............................................. R. dennisii (Figs 30−33)Conidia obovate to subglobose, 3-septate,with dark brown middle and basal cells,and pale brown at the apex, smooth,16−21 × 8−13 µm ..........................................................R. quadriloculare (Fig. 52)Conidia moniliform, 8−22-septate, brownand verrucose at the base and middle part,and pale brown and smooth towards theapex, 55−280 × 6−10 µm .................................................. R. moniliforme (Fig. 47)AcknowledgementsThe authors are grateful to Dr Lori Carris(Washington State University) for presubmissionreview of this paper. Gabriela Heredia,R.M. Arias and R.F. Castañeda Ruiz arethankful to CONABIO (Mexico) for financialsupport through project IE004. T. Iturriaga isgrateful to FONACYT, Venezuela, for financialsupport for project S1-2001000663. Theauthors thank Dr. Uwe Braun, Dr. Lori Carris,Dr. De-Wei Li, Dr. Felipe Wartchow, Dr.Antonio Hernández-Gutiérrez, Dr. Melissa282

MycosphereFigs 22−25 − Repetophragma spp. conidia.Redrawn from the original descriptions. 22 R.aburiense. 23 R. afrormosiae. 24 R. apiceinflatum.25. R. biseptatum. Scale bars = 10 µm.Figs 30−32 − Repetophragma dennisii, conidiophores,conidiogenous cells and conidia,photomicrographs from (USB C99/199). Scalebars 30, 31 = 10 µm, 32 = 20 µm.Figs 26−29 − Repetophragma spp. conidia.Redrawn from the original descriptions. 26 R.calongei. 27−28 R. cambrense. 29 R. cubense.Scale bar 27, 28 = 10 µm. 26, 29 = 20 µm.Figs 33−36 − Repetophragma spp. conidia.Redrawn from the original descriptions. 33 R.dennisii. 34 R. ellisii. 35 R. fasciatum. 36 R.filiattenuatum. Scale bar 33, 34, 35 = 10 µm,36 = 40 µm.283

Figs 37−40 − Repetophragma spp. conidia. Redrawn from the original descriptions. 37 R. filiferum,38 R. filisporum, 39 R. fragmentisporum, 40 R. goidanichii. Scale bar 37, 39, 40 = 10 µm, 38 = 20µm.Figs 41−44 − Repetophragma spp., conidia. 41 R. gondwanamycetis conidia. Adapted and redrawnfrom the original descriptions. 42 R. indicum. 43 R. inflatum, 44 R. laxisporum. Scale bars = 10 µm.284

MycosphereFig. 45 − Repetophragma laxisporum, photomicrographsfrom holotype (INIFAT C85/13).Conidiophores, conidiogenous cells and conidia.Scale bars = 10 µm.Fig. 46 − Repetophragma longiphorum, drawingof conidia from holotype (INIFAT C85/13). Scale bar = 10 µm.Figs 47−49 − Repetophragma spp., conidia. Redrawn from the original descriptions. 47 R. moniliforme.48 R. omahutaense. 49 R. ontariense. Scale bar 47, 49 = 40 µm, 48 = 10 µm.285

Fig. 50 − Repetophragma perproliferatum, drawing of conidia from holotype (INIFAT C91/10−4).Scale bar = 10 µm.Figs 51−54 − Repetophragma spp., conidia. Redrawn from the original descriptions. 51 R.peruamazonicum. 52 R. quadriloculare. 53 R. sinense. Scale bar = 10 µm. 54 R. subulatum. Scalebar 51, 52, 53 = 10 µm, 54 = 40 µm.286

MycosphereFig. 55 − Repetophragma subulatum. Conidiophores, conidiogenous cells and conidia, photomicrographsfrom (INIFAT C95/46). Scale bar = 10 µm.Figs 56 −58 − Repetophragma spp., conidia. Redrawn from the original descriptions. 56 R. terminaliae.57 R. wroblewskii. 58 R. zambiense. Scale bars = 20 µm.Mardones, Dr. Cony Decock, Dr. Shaun Pennycook, Dr. Walter Gams, Dr. Roland Kirschner,Dr. Sanjay K. Singh, Dr. Antonio Hernández-Gutiérrez, Dr. Xiu Guo Zhang, Dr. DJ Bhat, Dr.Sanjay K. Singh and Dr. Gregorio Delgado fortheir generous and valued assistance withliterature not otherwise available. We thankBeatriz Ramos, Mirtha Caraballo, Aliana Sosafor technical assistance. We also acknowledgethe facility provided by Dr. P.M. Kirk throughthe IndexFungorum website.287

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