Thermo Scientific Hypercarb Columns

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F OOD AND B EVERAGEApplication NotesFigure 2: Effect of coating concentration on retention behavior.The suitability of the method for quantitative analysiswas investigated through a linearity assessment. Calibrationcurves (Figure 3) were generated by plotting the peak responsearea against the concentration of the standards injected. Forfluoride and chloride 5 concentrations between 1 mg/L and10 mg/L were used. For bromide, nitrate, phosphate andsulphate concentrations between 10 mg/L and 100 mg/Lwere used. All analytes generate a true linear response overthe concentration ranges described, with the lowest R 2coefficient value being recorded at 0.9978 for phosphate.To evaluate the reproducibility of area and repeatabilityof retention times, the percentage relative standard deviation(RSD) values were recorded over ten and twenty injectionsrespectively (Table 1). The highest value observed for peakarea RSD was 0.5% for fluoride and the highest RSD valuefor retention time was 1.1%.The lifetime of the coated column was investigated bydoing repeated injections of the anion mixture. Retentiontimes remain constant for 445 runs, which equates to16000 column volumes of mobile phase through the column(Figure 4). As the coating process involves the use of 25%organic, it ensures that using 5% organic during analysis willnot be detrimental to column coating lifetime. The dynamiccoating of the PGC surface is reversible. Flushing the columnwith 100% organic will remove all column coating, andthe column can then be re-coated using original coatingconditions or used as a conventional Hypercarb column.Compound %RSD Peak Area %RSD Retention TimeFluoride 0.51 0.21Chloride 0.47 0.30Bromide 0.26 0.57Nitrate 0.20 0.75Phosphate 0.57 0.47Sulphate 0.27 1.09Table 1: Reproducibility: 10 repeated measurements for peak areas and20 repeated measurements for retention times.The methodology developed was applied to themeasurement of the level of inorganic anions present intwo brands on bottled water, as demonstrated in Figure 5.Figure 3: Linearity data in the concentration range of 10 to 100 mg/L for sulphate,nitrate, bromide and phosphate and 1 to 10 mg/L for fluoride and chloride.38
F OOD AND B EVERAGEFigure 4: Column coating lifetime. Reproducible retention times for 16000 column volumes of mobile phase through the column or 445 injections.Column: Hypercarb 5 µm, 100 x 4.6 mm (CTA 8 mM); Mobile phase: 2 mM Na 2 CO 3 /1 mM NaHCO 3 + 5% MeCN; other conditions as described in the experimental section.ConclusionDynamically CTA coated Hypercarb columns possess goodstability, reproducibility, linearity and lifetime performanceworking with organic compositions up to 25%. This approachoffers an alternative to the conventional methodology forthe determination on inorganic anions in drinking water.Application NotesReferences1. C. Elfakir, P. Chaimbault, M. Dreux, J. Chromatogr. A, 829 (1998) 1932. K. Petritis, P. Chaimbault, C. Elfakir, M. Dreux, J. Chromatogr. A, 1999,833, 1473. T. Takeuchi, T. Kojima, T. Miwa, J. High, Resol. Chromatogr., 2000, 23, 5904. T. Okamoto, A. Isozaki, H. Nagashima, J. Chromatogr. A, 1998, 800, 2395. H. Nagashima, T. Okamoto, J. Chromatogr. A, 1999, 855, 261-266Figure 5: Analysis of bottled drinking water.39
Page 1 and 2: Applications NotebookIssue 1, June
Page 3 and 4: Applications ReviewApplication Chro
Page 5 and 6: Retention Mechanisms on PGCOn a mol
Page 7 and 8: Porous Graphitic Carbon for the LC/
Page 9 and 10: the retention mechanism is a combin
Page 11 and 12: Data ProcessingThermo Scientific Xt
Page 13 and 14: Type of LC ColumnPeptides Graphite
Page 15 and 16: Higher-charged 4+ metal-adducted pr
Page 17 and 18: The Use of Porous Graphitic Carbon
Page 19 and 20: The lowest concentration from the d
Page 21 and 22: The experimental conditions for the
Page 23 and 24: Fast LC Separation of Triazine Herb
Page 25 and 26: SamplesSamples of surface water (Sa
Page 27 and 28: Analysis of Polar Metabolites of At
Page 29 and 30: Compounds Log Kow Recovery (%) S.DA
Page 31 and 32: ExperimentalSample PreparationFor t
Page 33 and 34: Determination of Leucine and its Is
Page 35 and 36: Determination of Occupational Expos
Page 37: Porous Graphitic Carbon for Inorgan
Page 41 and 42: Application Area Analyte Group Refe
Page 43 and 44: 69. J. Bessarda, P. Saviuc, Y. Chan
Page 45 and 46: Aflatoxinsafla1Nonylphenol Isomers1
Page 47 and 48: Ordering InformationHypercarb Colum

Thermo Scientific Hypercarb Columns

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