Thermo Scientific Hypercarb Columns

Application ChromatogramsAllantoinCyclic MonophosphatesL-CarnitineApplicationChromatogramsH350-1136Relative Abundance10095908580757065605550454035302520151050.0 0.5 1.0 1.5 2.0 2.5Time (min)Column: Hypercarb, 3 µm, 50 x 2.1 mmPart Number: 35003-052130Mobile Phase: A – H 2 O+ 0.1 % Formic acid;B – MeCN+ 0.1 % Formic acid.Isocratic: 95% A + 5% B, run for 5 min.Flow Rate: 0.3 mL/min.Detection: UV @ 205 nm; MS: ESI –ve,Temperature: 30 °C.Injection Volume: 5 µLH350-1133mAU1201008060402000 2.5 5 7.5Column: Hypercarb, 5 µm, 100 x 4.6 mmPart Number: 35005-104630Mobile Phase: A = 20 mM Ammonium Acetate(no pH modification)B = MeCNGradient: 20 – 45% B in 10 minutesFlow Rate: 1.0 mL/minDetection: UV @ 254 nmTemperature: 25 °CInjection Volume: 5 µL1. Cytidine 3', 5' – cyclic monophosphate2. Uridine 3', 5' – cyclic monophosphate3. Guanosine 3', 5' – cyclic monophosphate4. Adenosine 3', 5' – cyclic monophosphate1234minH350-1132Relative Abundance100959085807570656055504540353025201510Column: Hypercarb DASH HTS,5 µm, 20 x 2.1 mmPart Number: 35005-022150Mobile Phase: H 2 O + 0.1% TFAFlow Rate: 0.5 mL/minDetection: MS, +ve ESITemperature: 30 °CInjection Volume: 0.5 µL1. L-Carnitnine10.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4Time (min)RNB-GlycopeptidesPurines and Pyrimidines(UHT-LC)CeramidesH350-108007100301H350-1102Column: Hypercarb, 5 µm, 100 x 2.1 mmPart Number: 35005-102130Mobile Phase: A: MeOH44Column: Hypercarb, 5 µm, 100 x 4.6 mmPart Number: 35005-104646Mobile Phase: H 2 OFlow Rate: 2 mL/minDetection: UV at 254 nmTemperature: 150 to 200 °C at 15 °C/min;hold at 200 °C1. Cytosine 3. Thymine 5. Guanine2. Uracil 4. Hypoxanthine 6. XanthineB: CHCl 3Gradient: 45 to 80% B in 15 minFlow Rate: 0.4 mL/minDetection: ELSDTemperature: 50 °CSource:Ceramides:1. d18:1c16:02. d18:0c16:03. d18:1c18:04. d18:0c18:05. d18:1c22:1K. Gaudin, Laboratoire de ChimieAnalytique, Université ParisSud, France6. d18:1c20:07. d18:1c23:18. d18:1c24:19. d18:1c22:010. d18:1c25:111. d18:1c23:012. d18:1c26:113. d18:1c24:014. d18:1c25:015. d18:1c26:0Column: Hypercarb, 5 µm, 100 x 4.6 mmPart Number: 35005-104630Mobile Phase: A: H 2 OB: ACNGradient: 10 to 50% B in 50 minFlow Rate: 1 mL/minDetection: UV at 210 nmTemperature: 40 °CSource: J. Fan and A. Kondo, Anal.Biochem. 219, 224 (1994).Reproduced with permission1. R-I (Man 6 GlcNAc 2 Asn) 3. R-III (Man 6 GlcNAc 2 AsnLeu)2. R-II (Man 5 GlcNAc 2 Asn) 4. R-IV (Man 5 GlcNAc 2 AsnLeu)