Electrophoretic characterization of Amaranthus L. seed proteins and ...

SEED PROTEIN PROFILE OF AMARANTHUS 59Table 1. Localities and collection dates of the populations studiedSpeciesLocalities and voucher specimensSubgenus AmaranthusSection Amaranthus subsection AmaranthusA. retroflexus HUELVA. Lepe, 26.vii.1978, Silvestre (SEV 84403) (R-1). SEVILLA. LaLuisiana, 7.vii.2003, Juan & Pastor (215188) (R-2). Palomares delRío, 8.viii.2003, Fernández & Pastor (215189 (R-3)Section Amaranthus subsectionHibrida Mosyakin & K. R. RobertsonA. cruentus L. CÓRDOBA. Between Airport and Almodóvar del Río, 23.xi.1978,Muñoz & Ruiz de Clavijo (SEV 84640) (C-1). HUELVA. Higuera dela Sierra, 21.vii.1979, Rivera & Cabezudo (SEV 45758) (C-2).SEVILLA. Sevilla, Universidad Laboral, 25.xii.1973,Montero (SEV 20368) (C-3)A. hypochondriacus L. CÁDIZ. Algodonales, 30.ix.1978, A. Martínez (SEV 92681) (H-1).HUELVA. Between Gil Márquez and Valdelamusa, 21.vii.1979,Rivera & Cabezudo (SEV 45760) (H-2)A. powellii S. Watson ssp. bouchonii (Thell.) MADRID. Embalse de Puentes Viejas, 17.ix.1981 (SEV 113784) (P-1)Costea & CarreteroSubgenus Albersia (Kunth) Gren. & Godr.Section Blitopsis Dumort.A. blitum L. ssp. blitum SEVILLA. Sevilla, 30.vi.2003, Juan (SEV 215190) (Bl-1). La Luisiana,17.vii.2003, Juan & Pastor (SEV 215191) (Bl-2)A. deflexus L. CÓRDOBA. Los Pedroches, 6.viii.1976, Devesa (SEV 32852) (D-1).HUELVA.Galaroza, 30.viii.2003, Juan (SEV 215192) (D-2). SEVILLA. LaLuisiana, 17.vii.2003, Juan & Pastor (SEV 215193) (D-3)A. viridis L.CÓRDOBA. Córdoba, 18.viii.1978, Varela & González (SEV 83663)(V-1).HUELVA. Cartaya, viii.1979, Silvestre (SEV 83668) (V-2). SEVILLA.Sevilla, 30.vi.2003, Juan (SEV 215194) (V-3)Section Pentamorion (G. Beck) Mosyakin& K. R. RobertsonA. muricatus (Moq.) Hieron SEVILLA. Camas, 8.vii.2003, Juan & Pastor (SEV 215195) (M-1). LaLuisiana, 17.vii.2003, Juan & Pastor (SEV 215196) (M-2). San Juande Aznalfarache, 26.viii.2003, Juan (SEV 215197) (M-3)Section Pyxidium MoquinA. albus L. HUELVA. El Repilado, 17.viii.2003, Juan (SEV 215198) (A-1).SEVILLA. Camas, 8.vii.2003, Juan & Pastor (SEV 215199) (A-2). LaLuisiana, 17.vii. 2003, Juan & Pastor (SEV 215200) (A-3)A. blitoides S. Watson SEVILLA. Sevilla, 30.vi.2003, Juan (SEV 215201) (B-1). Camas,8.vii.2003, Juan & Pastor (SEV 215202) (B-2). La Luisiana,17.vii.2003, Juan & Pastor (SEV 215203) (B-3)A. graecizans L. ssp. sylvestris (Vill.) Brenan CÁDIZ. Grazalema, 28.ix.1984, Aparicio & Rowe (SEV 114345) (G-1)employing the Bray–Curtis index of dissimilarity(Bray & Curtis, 1957). The dissimilarity index wastransformed to the index of similarity (1 - dissimilarityindex ¥ 100). A dendrogram was produced from thedistance matrix using the unweighted pair groupmethod with arithmetic average (UPGMA).RESULTS AND DISCUSSIONAccording to the Osborne classification, Amaranthusseed proteins are composed of albumins, globulins,and glutelins in similar proportions and prolamins inminor amounts (Abugoch, Martínez & Añón, 2003).© 2007 The Linnean Society of London, Botanical Journal of the Linnean Society, 2007, 155, 57–63