Summary - People.stat.sfu.ca - Simon Fraser University

Table 1. Summary of sampling protocols for the experiments conducted.Environment Canada StudiesAquatic species Response Sampling protocol at each combination of waterhardness and sulphate levelsRainbow TroutChinookHyalellaMusselsBullfrogtadpolesFat headminnowsLemnaDaphniaRotiferFat headminnowsSurvival of eggs to21 daysSurvival of eggs to28 days.Survival andgrowth oforganisms to 28days.Survival andgrowth oforganisms to 28days.Survival andgrowth to 28 days.Survival andgrowth to 7 days.Frond growth andincrease in weightSurvival for 6 daysand reproductionReproduction after49 hours.Survival andgrowth to 7 days.Triplicate batches of 30 eggs were incubated andthe number of mortalities from each batch wasrecorded.Triplicate batches of 30 eggs were incubated andthe number of mortalities from each batch wasrecorded.Quintuplicate batches (except for 10 batches inthe case of control doses of sulphate in softwater) of 15 Hyalella were incubated and thenumber of mortalities from each batch wasrecorded. The mean weight of each batch of theorganisms at the end of the experiment wasmeasured.Triplicate batches of 3, 3, or 4 mussels wereincubated and the number of mortalities in eachbatch was recorded. Wet weight and thebeginning and end of the experiment wasmeasured.Triplicate batches of 5 tadpoles were incubatedand the number of mortalities in each batch wasrecorded. The change in weight over the 28 dayswas also recorded.Quadruplicate batches of 10 minnows wereincubated and the number of mortalities in eachbatch was recorded. The final mean weight ineach batch was also recorded.Quadruplicate replicates of Lemna wereincubated and the number of new fronds andfinal weight were recorded for each survivingorganism.Nautilus Studies10 individual organisms were incubated and thestatus (dead/alive) and reproductive output wasrecorded.8 individual organisms were incubated and thepopulation growth was recorded.Triplicate batches of 10 minnows were incubatedand the number of mortalities was recorded. Thefinal mean weight in each batch was alsorecorded.