Technical guidelines on testing the migration of primary aromatic ...

(1.2.2.4) or sulfuric acid solution (1.2.2.9), as appropriate. Add 5 ml of pentane-2,4-dione reagent (2.2.1.2) and continue as described in sample treatment.2.3.1.2 For the determination of formaldehydeTransfer into a 50 ml flask 5,0 ml of aqueous food simulant, add 20,0 ml water and5,0 ml of pentane-2,4-dione reagent and continue as described in sample treatment.2.3.2 Preparation of blank samplesTreat simulants that have not been in contact with packaging material, as describedin sample preparation.2.3.3 Preparation of calibration samplesTransfer into a 50 ml flask 5 ml of diluted standard solutions, 20 ml water and add5.0 ml of pentane-2,4-dione reagent.2.3.4 Reference solutionTransfer into a 50 ml flask 5,0 ml aqueous food simulant, 20 ml water and 5,0 ml ofthe reagent without pentane-2,4-dione.NOTE: Possible interferences due to background colour in the test sample are eliminated by theuse of this reference solution.2.3.5 Sample treatmentShake the mixtures prepared. Immerse the flasks in a waterbath at 60 °C for exactly10 min. Allow to cool for 2 min in a bath of iced water. Within 25 min from themoment when the flasks are placed in the water-bath measure the absorbance at410 nm of the sample solution with the reference solution in the reference cell.2.4 Evaluation of dataEvaluate the data following the procedure given for protocol A.55