Pharm D Revised Regulations 2008 - Rajiv Gandhi University of ...

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36Theory : 3 Hrs. /Week2.2 PHARMACEUTICAL MICROBIOLOGY (THEORY)1. Scope of the Subject: Microbiology has always been an essential component ofpharmacy curriculum. This is because of the relevance of microbiology to pharmaceuticalsciences and more specifically to pharmaceutical industry. Pharmaceutical biotechnologyis the logical extension of pharmaceutical microbiology, which is expected to change thecomplete drug product scenario in the future.This course deals with the various aspects of microorganisms, its classification,morphology, laboratory cultivation identification and maintenance. Its also discusses withsterilization of pharmaceutical products, equipment, media etc. The course furtherdiscusses the immunological preparations, diseases its transmission, diagnosis, controland immunological tests.2. Objectives of the Subject :Upon completion of the subject student shall be able to –a. know the anatomy, identification, growth factors and sterilization of microorganisms;b. know the mode of transmission of disease causing microorganism, symptoms ofdisease, and treatment aspect;c. do estimation of RNA and DNA and there by identifying the source;d. do cultivation and identification of the microorganisms in the laboratory ;e. do identification of diseases by performing the diagnostic tests; andf. appreciate the behavior of motility and behavioral characteristics of microorganisms.Text books (Theory)a. Vanitha Kale and Kishor Bhusari ― Applied Microbiology HimalayaPublishinghouse Mumbai.b. Mary Louis Turgeon ― Immunology and Serology in Laboratory Medicines2 nd edition, 1996 Mosby- Year book inc St. Louis Missouri 63146.c. Harsh Mohan, ― Text book of Pathology 3 rd edition, 1998, B-3 Ansari roadDarya ganj N. Delhi.Reference books (Theory)a. Prescot L.M., Jarley G.P Klein D.A ―Microbiology 2 nd - edition Mc GrawHillCompany Incb. Rawlins E.A.Bentley’s Text Book of Pharmaceutics B ailliere Tindals 24-28London 1988c. Forbisher ― Fundamentals of Microbiology Philidelphia W.B.Saunders.d. Prescott L.M. Jarley G.P., Klein.D.A. ― Microbiology.2 nd edition WMCBrownPublishers, Oxford. 1993e. War Roitt, Jonathan Brostoff, David male, ― Immunology3 rd edition 1996,Mosby- year book Europe Ltd, London.f. Pharmacopoeia of India, Govt of India, 1996.
373. Detailed syllabus and lecture wise schedule :Title of the topic1 Introduction to the science of microbiology. Major divisions of microbialworld and Relationship among them.2 Different methods of classification of microbes and study of Bacteria,Fungi, virus, Rickettsiae, Spirochetes.3 Nutritional requirements, growth and cultivation of bacteria and virus.Study of different important media required for the growth of aerobic andanaerobic bacteria & fungi. Differential media, enriched media andselective media, maintenance of lab cultures.4 Different methods used in isolation and identification of bacteria withemphasis to different staining techniques and biochemical reactions.Counting of bacteria -Total and Viable counting techniques.5 Detailed study of different methods of sterilization including their meritsand demerits. Sterilization methods for all pharmaceutical products.Detailed study of sterility testing of different pharmaceutical preparations .Brief information on Validation.6 Disinfectants- Study of disinfectants, antiseptics, fungicidal and virucidalagents factors affecting their activation and mechanism of action.Evaluation of bactericidal, bacteristatic, , virucidal activities, evaluation ofpreservatives in pharmaceutical preparations.7 Immunology- Immunity, Definition, Classification, General principles ofnatural immunity, Phagocytosis, acquired immunity( active and passive ) .Antigens, chemical nature of antigens structure and formation ofAntibodies, Antigen-Antibody reactions. Bacterial exotoxins andendotoxins. Significance of toxoids in active immunity, Immunizationprogramme, and importance of booster dose.8 Diagnostic tests : Schick’s Test, Elisa test, Western Blot test, Southern BlotPCR Widal, QBC, Mantaux Peripheral smear. Study of malarial parasite.9 Microbial culture sensitivity Testing: Interpretation of results Principlesand methods of different microbiological assays, microbiological assay ofPenicillin, Streptomycin and vitamin B 2 and B 12 . Standardisation ofvaccines and sera.10 Study of infectious diseases: Typhoid, Tuberculosis, Malaria, Cholera,Hepatitis, Meningitis, Syphilis & Gonorrhea and HIV.2.2 PHARMACEUTICAL MICROBIOLOGY (PRACTICAL)Practical : 3 Hrs./WeekTitle of the Experiment:1 Study of apparatus used in experimental microbiology*.2 Sterilisation of glass ware’s. Preparation of media and sterilisation.*3 Staining techniques – Simple staining ; Gram’s staining ; Negative staining**4 Study of motility characters*.5 Enumeration of micro-organisms (Total and Viable)*6 Study of the methods of isolation of pure culture.*7 Bio chemical testing for the identification of micro*-organisms.
Page 1 and 2: 1RAJIV GANDHI UNIVERSITY OF HEALTH
Page 3 and 4: 3Second Year:S.No Name of Subject N
Page 5 and 6: Sixth Year:5Internship or residency
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Page 9 and 10: 12. Declaration of Class9Class shal
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Page 17 and 18: Theory : 2 Hrs. /Week171.2 PHARMACE
Page 19 and 20: 195. Linimentsa. Liniment of turpen
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Page 35: 35- Amylodosis4 Cancer: differences
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Page 41 and 42: 41Theory : 3 Hrs. /Week2.4 PHARMACO
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Page 67 and 68: 67Practical : 3 Hrs./Week4.1 PHARMA
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Page 83 and 84: 83APPENDIX-BCONDITIONS TO BE FULFIL
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874. Director orPrincipal orHead of
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895) ACCOMMODATION :Suitable and su
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91II. Apparatus:S.No Name Minimum r
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938 Muffle Furnace 019 Mechanical S
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9537 Conical Percolator (glass/copp
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97F. DEPARTMENT OF PHARMACY PRACTIC
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99APPENDIX-CINTERNSHIP1) SPECIFIC O
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