B.Tech. Biotechnology (Molecular & Cellular ... - Shiats.edu.in

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Unit 3. Direct gene transfer in plants: Physical (Particle gun delivery,electroporation, microinjection, macroinjection, electroinjection, fiber mediated DNAdelivery, Laser induced DNA uptake, Sonication) and Chemical methods of genetransfer (Poly ethylene glycol, Poly vinyl alcohol, Calcium phosphate)Unit 4. Applications of plant biotechnology: Improving agronomic traits - geneticmanipulation of plants for salt resistance, herbicide resistance, fungi and virusresistance, insect and other pest resistance. Modification of production traits -delayed fruit ripening, improving seed storage proteins.PracticalsSafety aspects and precautions to be taken in Biotechnology LaboratoryPreparation of reagents, stock solutions and buffers for plant DNA isolation.Study of Labwares used for isolation of DNAIsolation of genomic DNA from plant tissues by CTAB method.Purification of crude DNA samples.JSBB 400 TRAINING I I (0-0-1)
5 th SemesterECE-430 ELECTRONIC MEASUREMENT & INSTRUMENTATION 3(2-0-1)Semiconductors: energy band theory of solids, concept of solids, concept offorbidden gaps, insulators and semiconductors. Transport phenomenon insemiconductors, instridic, and extrinsic semiconductorsJunction diode p-n junction diode vi characteristics, diode application as arectifier (half wave and full wave) breakdown mechanism –se4len and Avalenchecharacteristics, zener diode and its applications LED and its applicationsBijunction transistor, CE, CB Configuration, characteristics, curves, requirementof biasing, stability, small signal equivalent ckt, h- parameter model, transistor asan amplifier, frequency response of amplifiers, negative feed back, gain bandwidth product, oscillators and condition for oscillationOther semiconductor devices: introduction of JFET, MOSFET, and CMOSSwitching theory and logic gates number systems, Boolean algebra, logic gates,flip flop and ICSA/D and D/A converters introduction, methods of conversion A/D, D/AQuantities of measurements: introduction, performance characteristics, staticcharacteristic, error in measurement, types of error, and source of error, dynamiccharacteristicElectronic instruments: ammeter, voltmeter, ohm meter, VOM, Q meterCRO block diagram and workingTransducers and measurements of electrical and non electric quanta ties:measurement of liquid level, flow temperature, strain, pressure, force, torque,power, displacement, vibration, acceleration due to transducers. Measurement ofresistance, inductance, capacitance using DC (Wheatstone bridge) and AC(MAXWELL Bridge, WEINBRIDGE) bridges.List of Practicals:Study of diode characteristics
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