B.Tech. Biotechnology (Molecular & Cellular ... - Shiats.edu.in

7 th SemesterMCE 601 MOLECULAR BREEDING 3 (2-0-2)Unit 1. Introduction to markers: Different kinds of markers- morphological,biochemical and molecular markers.Unit 2. Molecular marker systems: PCR based approaches - RAPD, SSR, AFLP,AP-PCR; targeted PCR, STS, SCAR; and non-PCR based approach (RFLP).Unit 3. Marker assisted selection (MAS): Development of markers, generation ofmapping population, bulk segregate analysis, use of NILS for tagging genes,selection of QTLS for MAS.Unit 4. Molecular markers in plant breeding: Uses of molecular markersresistancebreeding, gene interogression, gene pyramiding, antibiotic resistance,improvement of qualitative characters, fertility restorer genes and hybrid vigour.PracticalsExtraction of proteins from plant tissuesProtein profiling of crop plants by Polyacrylamide gel electrophoresisStudy of Labwares used in PCR analysisPCR amplification of DNA samplesElectrophoretic separation of PCR productsStaining, visualization and scoring of PCR productsMCE 602 GENOMICS 3 (3 - 0- 0)Unit 1. Introduction: Basic concepts of genomics. Structural and functionalgenomics. Proteomics, transcriptomics, metabolomics and phenomics.Unit 2. Genome: Physico-chemical properties of genome, genome size andsequence components, gene structure and higher order genome organization.Unit 3. Genome analysis: DNA sequencing, DNA Micro array, gene finding,genome databases and data mining. Structural, functional and comparativegenomics.Unit 4. Genome mapping: Basic concepts, model organisms, genome projects,genetic and linkage map, cytogenetic maps, physical maps, comparative genomemapping.MCE 603 GENETICALLY MODIFIED FOODS 3 (3-0-0)