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Inactivation of E. <strong>coli</strong> <strong>in</strong> UCFMTable of ContentsTerms of Reference...........................................................................................4Summary and Recommendations....................................................................5Acknowledgments.............................................................................................71. Introduction...............................................................................................82. Pathogenic Escherichia <strong>coli</strong> and UCFM..................................................92.1 Background ......................................................................................92.2 Regulatory Responses .....................................................................93. Uncooked Fermented Meat Processes .................................................103.1 History ............................................................................................103.2 Mak<strong>in</strong>g UCFM ................................................................................104. Non-thermal <strong><strong>in</strong>activation</strong> of Escherichia <strong>coli</strong> .......................................184.1. Introduction ....................................................................................184.2 Ecophysiology of Escherichia <strong>coli</strong> ..................................................184.3 Non-thermal <strong><strong>in</strong>activation</strong> k<strong>in</strong>etics <strong>in</strong> laboratory broth systems........194.4 K<strong>in</strong>etics of Inactivation dur<strong>in</strong>g UCFM Production ...........................225. Modell<strong>in</strong>g of Inactivation Data ...............................................................315.1 Temperature...................................................................................315.2 pH...................................................................................................365.3 Water activity..................................................................................365.4 Other - Role of Oxygen ..................................................................375.5 Conclusions from available studies ................................................376. Model Systems: Relevance to Studies <strong>in</strong> Commercial UCFMProcesses ........................................................................................................396.1 Comparison of model broth systems and UCFM............................396.2 Presence of Fat..............................................................................406.3 Inoculum Preparation and Prior History..........................................416.4 Other Variables ..............................................................................436.5 Conclusions....................................................................................437. Conclusions and Recommendations....................................................447.1 Introduction ....................................................................................447.2 Gaps <strong>in</strong> current knowledge.............................................................447.3 Recommendations to Acquire Data................................................457.4 Other Recommendations ...............................................................47References.......................................................................................................49Page 2 of 59

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