eman - NCMI

More documents

Recommendations

Info

Particle Picking (Boxing)●●●●●●cd ~/data/<strong>eman</strong>.demo/GroEL/stage1lscat 000scriptboxer < one of the mrc files>try Boxes->Autobox after picking 3-4 particlese2boxer.py < mrc file> --box= 128 --guiWhen you get bored ..../000scriptparticles will now be in ../stage2
Particle Picking (Boxing)●●cd ~/data/<strong>eman</strong>.demo/samples/boxerboxer Tricks:●●makeboxref.py groel.mrc sym=d7 invert ang=15(creates a set of reference images from a 3D model for autoboxing)batchboxer input=jj5339.f.mrc auto=.3,.7,.1 dbout=5339.boxoutput=5339.ptcl.hed refimg=best.hed(autoboxes a single micrograph, parameters determined in boxer,refimg specifies reference particles for boxing)
Page 1 and 2: TutorialSession
Page 3 and 4: EMAN2 Documentation●●●●●A
Page 5 and 6: GUI Programs● eman● v2● boxer
Page 7 and 8: Canonical EMAN Refinement●●●
Page 9: Canonical EMAN Refinement●●●
Page 17 and 18: First Preliminary ModelLook at:●
Page 19 and 20: Basic Image Processing● iminfo [
Page 21 and 22: CTF Correction EMANInitial3D Mod
Page 23 and 24: CTF Parameter Determination●●fi
Page 27 and 28: Refine Options●Projection:mask=pr
Page 29 and 30: Refine OptionsREQUIRED:●●●
Page 31 and 32: Refine OptionsOPTIONAL:●●filt3d
Page 33 and 34: Refine OptionsTYPICAL (not required
Page 39 and 40: 2D Analysis Step 2(particle extra
Page 41 and 42: multirefine●●●●No demo data
Page 43 and 44: multirefine●●When multirefine c
Page 45: Generating a Hybrid Structure Facto

eman - NCMI

Create successful ePaper yourself

Delete template?

Save as template?