eman - NCMI

Refine OptionsREQUIRED:●●● ­ Final number of iterationsyou wish to be complete in thisdirectory. ie ­ if 5 are complete and youspecify 6, 1 more iteration will runmask= ­ A circular mask to beapplied virtually everywhere. Should bea few pixels larger than the largestradius of your particlectfc=, ctfcw= OR median ­ CTFcorrection options. ctfc takes a filterresolution in Angstroms, but ctfcw is farsuperior. ctfcw takes the name of a 1Dstructure factor file used when fitting thedata (MUST be the same file). mediandoes no CTF correction at all. Naturallythe data must be properly preprocessedfor this option to function, otherwisecrashes or invalid results are likely.●●●hard= ­ Rather obscure. Thisoption determines when a class­averageshould be excluded from the 3Dreconstruction process. 25 is generallygood. see 'make3d' for more info.sym= ­ For asymmetricobjects, either omit this specification, oruse 'c1'. cn denotes a single n­foldrotational symmetry (about the z­axis),dn denotes n­fold dihedral symmetry (cnwith n 2­folds in the x­y plane), oct isoctahedral (2­3­4, symmetry of a cube),icos is icosahedral (2­3­5).ang= ­ Angular spacing betweenprojections. Smaller numbers producemore projections, usually between 1­10.Usually 90/n where n is any integer.