How to handle liver biopsy specimens

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Orcein staining: requirements • Optimal fixation and processing (Folds, scratches dark brown) • Adequate pre-staining oxidation (renew KMNO4 weekly) = same as that used for reticulin) • pH of orcein solution
Orcein • Hepatitis B surface antigen Homogeneous cy<strong>to</strong>plasmic inclusions • Low sensititvity ( ↓ in buffer formalin) • Does not detect membranous deposition • Elastic tissue • Age of areas of collapse → not very useful) • Vascular thrombi
Page 1 and 2: Liver Biopsy in the Assessment of M
Page 3 and 4: Liver Biopsy - Processing / Interpr
Page 5 and 6: Liver biopsy Specimen collection /
Page 7 and 8: Handling the biopsy specimen Essent
Page 9 and 10: Routine slides x 8 (2 held unstaine
Page 11 and 12: NRH
Page 13 and 14: Active Inactive
Page 15: PAS after diastase Performed routin
Page 19 and 20: Copper handling by hepatocytes Caer
Page 21 and 22: Copper / Copper-associated protein
Page 23 and 24: Perls’ for iron Ferritin + haemos
Page 25 and 26: Immunohistochemistry • Numerous c
Page 27 and 28: HBV Vertical transmission HBc tissu
Page 29 and 30: Liver allograft CMV (microabscess)
Page 31 and 32: CK7 Chronic cholestasis / CK7 Chron
Page 33 and 34: Progressive familial intrahepatic c
Page 35 and 36: Electron microscopy • Requires 1m
Page 37 and 38: Additional clinical information - P
Page 39: King’s College Hospital Institute

How to handle liver biopsy specimens

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