BIOT

3:00 130. Variable surface transport modalities on functionalized chromatographic supports revealed with
single molecule spectroscopy. L.J. Tauzin, H. Shen, J.K. Rasmussen, C. Bothof, G. Griesbraber, A.K.
McNulty, C.F. Landes
3:20 Intermission.
3:40 131. Probing nanoscale effects of ligand density in tentacular ion exchangers using x-ray scattering. R.S.
Bhambure, D.G. Greene, C. Gillespie, M.W. Phillips, H. Graalfs, A. Rapp, A.M. Lenhoff
4:00 132. Application of mechanistic modeling for enabling PAT in process chromatography: Separation of
charge variants of mAbs by ion exchange chromatography. V. Kumar, A.S. Rathore
4:20 133. Spectral deconvolution of chromatograms with PLS models and calibration-free methods. M.
Rüdt, N. Brestrich, J. Hubbuch
4:40 134. General gradients for preparative chromatography. A. Holmqvist, A. Sellberg, F. Magnusson, B.
Nilsson, A. Staby
Westin San Diego
Diamond I&II
Biomolecular & Biophysical Processes
Protein Engineering & Design
Section C
B. Hackel, J. Kaar, H. Samra, Organizers
A. J. Karlsson, D. Liu, Organizers, Presiding
2:00 135. When bad is good: Directed evolution using negative selection can result in alleles with superior
properties. B. Steinberg, M. Ostermeier
2:20 136. Creating catenanes with lasso peptides. C.D. Allen, A. Link
2:40 137. Yeast surface display-based method for the directed evolution of optimized self-cleaving intein
purification tags. S.D. Stimple, M.J. Coolbaugh, Y. Fan, K. Cochran, D.W. Wood
3:00 138. Computationally de novo designed peptide-mediated hetero-oligomeric interactions for modular
self-assembly of protein and lipoprotein nanostructures. M.S. Ardejani
3:20 Intermission.
3:40 139. Functional selection of full-length antibodies in the cytoplasm of living bacterial cells. M.
Robinson, N. Ke, M. Berkemen, M.P. DeLisa
4:00 140. Improved methods for co-evolving the affinity and stability of antibody fragments specific for
amyloid-forming polypeptides. K.E. Tiller, M. Julian, C. Lee, L. Rabia, J. Young, P.M. Tessier
4:20 141. Engineering antibody specificity through multi-dimensional high-throughput screens. D. Li, Y.
Cho