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248. Characterizing oxygen kLa in bioreactors to support scale-up and scale-down of cell culture bioprocesses. G. Yao, S.R. Patel, Z. Ahmed, L. Marquardt, J. Lagos, K. Aron, J. Yee, M.C. Borys, Z. Li 249. Gamma irradiated microcarrier-based virus production in a single-use rocking bioreactor system. A. Magnusson, M. Lundgren, E. Blanck, M. Berg 250. Implementing automated sampling, process monitoring, and nutrient feedback control for a system of 3- L bioreactors. K. O'Neill, A. Mehta, L. Hoshan, S. Xu, T. Seamans 251. Enhanced settling for single-use clarification. S. Elouafiq, A. Brinkmann, S. Tingen, M. Westoby 252. Evaluating the cytotoxicity of antioxidant and plasticizer additives used in single use cell culture bags. R. Shah, T. Linville, L. Yordy, J. Briggs, N. Chandarana, A. Whynot, C.S. Brazel 253. Development of a model at home medical device using a two-step enzymatic process for the detection of hemoglobin A1c levels in human blood. N. Ledra, T. McCaffrey, J. Cabrera 254. Effect of surfactants on the fluorescence behavior of dye-conjugated DNA for FRET enhancement. T. Oh, J. Choi, M. Heller 255. Synthesis of CdSe/ZnS nanocrystals and their bioconjugation to DNA. C.D. Hanson, L. Mohror 256. Simultaneous detection of Vibrio cholerae and cholera toxin using a hybrid chip ba double biomolecular markers. H. Shin, J. Seo, C. Kim, B. Hwang, H.J. Cha 257. Creating a DNA thermometer: An application for DNA computation. R.J. Karadeema 258. Micromachined multielectrode microprobes for glutamate and dopamine detection. A. Yorita, H. Monbouquette 259. Multiplexed detection of pathogenic DNA using zinc finger proteins without DNA amplification. J. Kim, M. Kim 260. Novel applications to the new development of amperometric biosensor for serum cholesterol level determination. Y. Lo, E.J. Parish, H. Honda, T. Wei 261. Molecular basis of unidirectional walking motion in Myosin-V. S. Mukherjee, A. Warshel 262. Novel application of scanning electron microscopes and the comparison of non-native Tephritid flies. E.J. Parish, Y. Lo, H. Honda, T. Wei 263. Process analytical technology solutions for real-time monitoring and control of CHO bioprocesses. E.R. Gibson 264. Withdrawn. 265. Strategies for optimizing a cell culture platform to achieve high recombinant protein titer. R. Gruver, S.B. Varma, N. Vijayasankaran, S.J. Meier 266. Improving the dynamic control of fatty acid ethyl ester production. A. Yaguchi, E. Arvay, G.M. Rodriguez, N. Wilson, M.A. Blenner
267. Gamma-aminobutyric acid production through GABA shunt by the introduction of synthetic scaffolds in recombinant Escherichia coli. S. Hong, V. Pham, S. Somasundaram 268. Genetic incorporation of unnatural amino acids biosynthesized from α-keto acids by an aminotransferase. W. Ko, H. Lee 269. Genetic incorporation of recycled unnatural amino acids. H. Lee, S. Kim 270. Translating unnatural amino acids with phenotypically-diverse, engineered EF-Tu and tRNA variants. V. Cox, E. Gaucher 271. Developing a protein-specific purification approach for osteopontin expressed in chloroplast of Chlamydomonas reinhardtii. A. Ravi, S. Guo, S. Kulkarni, B. Rasala, M. Tran, S. Mayfield, Z.L. Nikolov 272. Synthetic biology based multiple approaches for coenzyme B12 production in Escherichia coli. M. Noh, H. Lim, S. Park, G. Jung 273. Overcoming cellular barriers for enhancing polymer-mediated transgene expression. M. Christensen, J. Elmer, S. Barua, S. Eaton, L. Gonzalez-Malerva, J. Lehrman, J. LaBaer, K. Haynes, K. Rege 274. Development of advanced genome engineering tools for a model cyanobacterium. H.R. Aucoin, M. Sanktjohanser, N.R. Boyle 275. Functional chitosan-nanofibers fabricated by an acidic shell matrix protein for the preparation of efficient tissue engineering scaffold. W. Song, Y. Choi 276. New approaches to optically transparent elastic materials for optical device in ophthalmology. Y. Lo, H. Shyu, H. Honda, T. Wei 277. Particle size evaluation for improved CHO and E. coli harvest operations. S. Gerepka, J. Bill, A. Naim, J. Borrajo 278. Dendronized polymers for mRNA delivery. N.J. Oldenhuis, Z. Guan 279. Engineering large pore mesoporous silica nanoparticles for progene delivery application. H.W. Omar, J.G. Croissant, L. Deng, N.M. Khashab 280. Design and formulation of novel nanoemulsions that inhibit Mycobacterium smegmatis biofilm formation. A. Kratzer, M. Brantley, C. Mageeney, W. Magilton, V. Ware, B. Berger 281. Novel preparation and characterization of Epicatechin-3-allopyranoside. E.J. Parish, J. Wu, H. Ho, H. Honda, T. Wei 282. Novel development of a computational drug target prediction method based on chemical similarity networks. Y. Lo, E.J. Parish, H. Honda, T. Wei 283. Determination of optimum bead size for immobilized cell fermentations for enhanced production of lovastatin by Aspergillus terreus. G. Chun 284. Development of of fed-batch fermentation process for enhanced production of cis-cis muconic acid using high-yielding mutant cells of Corynebacterium glutamicum. S. Kim, G. Chun, S. Byun, S. Seo, B. Lee, S. Park, Y. Kim, W. Shin, S. Lee, E. Kim, D. Lee, S. Kim
Page 1 and 2: BIOT Division of Biochemical Techno
Page 3 and 4: 9:10 19. Imaging the oxidation stat
Page 5 and 6: 4:00 39. Case study on the producti
Page 7 and 8: 3:40 61. Genome-scale engineering i
Page 9 and 10: 11:10 80. Cell death during product
Page 11 and 12: 10:10 100. Comprehensive bioconvers
Page 13 and 14: Microbial Process Development M. R.
Page 15 and 16: 4:40 142. Evolving an intrinsically
Page 17 and 18: 242-244, 246-248, 250-253, 255-258,
Page 19 and 20: 9:10 177. Rethinking affibody: Evol
Page 21 and 22: 8:30 196. Exploring enhancement of
Page 23 and 24: 4:00 217. Optimization of mAb charg
Page 25: 3:40 238. Application biophysical t
Page 29 and 30: 302. Inference of cohort-specific H
Page 31 and 32: 338. Chromatographic depth filters:
Page 33 and 34: 373. Developmental approaches for o
Page 35 and 36: 409. Mapping the collective respons
Page 37 and 38: Section C Westin San Diego Diamond
Page 39 and 40: 11:05 455. Use of quality by design
Page 41 and 42: 4:00 476. Investigation of low mole
Page 43 and 44: 2:20 496. Process linkage approach
Page 45 and 46: 10:10 515. Benzonase ® endonucleas
Page 47 and 48: 10:10 Panel Discussion. Section F W
Page 49 and 50: 2:00 554. Salt tolerant tangential

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